摘要
对刺芹侧耳(Pleurotus eryngii)4个双核菌株的8个原生质体单核体进行基因组重测序和交配型位点的生物信息学分析,并对单核体之间的亲和性进行遗传分析。结果表明:8个单核体中含有3种A交配型位点和4种B交配型位点;其中3种A交配型位点都包含一对HD 1和HD 2基因,以典型的‘头对头’方式排列,HD基因两端有保守mip和β-fg基因;4种B交配型位点的信息素受体和信息素前体的数量及在基因组中的分布上差异较大,表明B交配型位点存在更丰富的遗传多样性。
Protoplasts of eight monokaryons segregated from four dikarytotic Pleurotus eryngii strains were subjected to whole genome re-sequencing,bioinformatics analysis of their mating-type loci and genetic analysis of affinity between these monokaryons.The results showed that there were three A mating-type loci and four B mating-type loci in these monokaryons.All three A mating-type loci contain a pair of HD 1 and HD 2 genes arranged in a typical'head-to-head'manner,and are closely flanked by conservative genes mip and β-fg at two ends,respectively.There are large variations in the number of pheromone receptor and pheromone precursor genes at the four B mating-type loci and their distribution in the genome,suggesting a high genetic diversity in B mating-type loci.
作者
徐思佳
李燕
万佳宁
杨瑞恒
唐利华
龚明
鲍大鹏
尚俊军
XU Sijia;LI Yan;WAN Jianing;YANG Ruiheng;TANG Lihua;GONG Ming;BAO Dapeng;SHANG Junjun(College of Food Science,Shanghai Ocean University,Shanghai 201406,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture and Rural Affairs,P.R.China,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungi Processing,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China)
出处
《食用菌学报》
CSCD
北大核心
2021年第3期19-26,共8页
Acta Edulis Fungi
基金
上海市科学技术委员会科技支撑重点攻关项目(17391900400)
上海市农业科学院食用菌研究所预研究项目(2021年度)。
关键词
刺芹侧耳
双核菌株
原生质体单核体
交配型位点
Pleurotus eryngii
dikaryotic strain
monokaryotic protoplast
mating-type locus
