人工合成松乳菇菌根及杂菌侵染宿主的形态学观察和分子鉴定

Morphological Observation of Synthesized Ectomycorrhizae betweeen Lactarius deliciosus and Three Pinus Species and Molecular Identification of Contaminating Species

将松乳菇(Lactarius deliciosus)菌丝块人工接种于意大利石松(Pinus pinea)、辐射松(P.radiata)、华山松(P.armandii)3种宿主植物的幼苗根部合成外生菌根,结果表明:松乳菇与意大利石松、辐射松、华山松均可形成外生菌根,菌根呈二叉状分支,分支末端呈棒状,整体呈鲜黄色至橘黄色。在合成松乳菇菌根过程中发现,杂菌1号可与意大利石松形成外生菌根,菌根呈二叉状分支,分支端细长,呈冠状膨大,菌套表面菌丝呈卷毛状,赭色至黄棕色,颜色随着时间变深棕色;杂菌2号可与华山松形成外生菌根,菌根呈二叉状分支,末端呈球状,浅黄色且半透明状。经ITS序列鉴定杂菌1号为Sphaerosporella sp.,杂菌2号为Peziza ostracoderma。

Seedlings of three pines,Pinus pinea,P.radiata and P.armandii were infected with mycelial blocks of Lactarius deliciosus to form ectomycorrhizae.The results showed that all three pines developed ectomycorrhizae with L.deliciosus.The synthesized ectomycorrhizae were bifurcated with rod-shaped tips,and the color was bright yellow to orange.During the ectomycorrhizal synthesis experiment,two contaminating fungi,named as No.1 and No.2,also formed ectomycorrhizae with P.pinea and P.armandii,respectively.The ectomycorrhizae formed by No.1 and P.pinea were bifurcated with spindly tips,coronally swollen,ochre to yellowish brown in color which turned dark brown over time,and had curly hyphae on the surface of the sheath.The ectomycorrhizae formed by No.2 and P.armandii were bifurcated with spherical tips,pale yellow and translucent.Analysis of ITS sequences showed that No.1 and No.2 were Sphaerosporella sp.and Peziza ostracoderma,respectively.