人参虫草菌质正丁醇部位化学成分研究

Chemical Constituents of n-butanol Fraction of Ginseng Cordyceps Fungal Substance

目的按照优选的生物转化工艺对人参进行生物转化,得到全新产物人参虫草菌质,对其正丁醇部位进行化学成分研究,阐明其药效物质基础。方法采用D101大孔树脂柱色谱,MCI-gel柱色谱,ODS柱色谱和半制备高效液相色谱仪对人参虫草菌质正丁醇部位进行分离纯化,根据理化性质以及氢谱(^(1)H-NMR)和碳谱(^(13)C-NMR)数据鉴定化合物结构。结果从人参虫草菌质正丁醇部位分离并鉴定出6个化合物,分别为人参皂苷Rh21(1)、人参皂苷Re_(3)(2)、人参皂苷Rg_(1)(3)、人参皂苷Re(4)、人参皂苷Rc(5)和人参皂苷Rb_(1)(6)。结论以上化合物均为首次从人参虫草菌质中分离得到,其中化合物1在人参原药材中未见报道,证明生物转化技术能改变人参的化学成分,为人参药材的二次开发利用提供参考。

Objective The ginseng was bio-transformed under the optimal condition to obtain the ginseng cordyceps fungal substance(GCFS).This study was conducted to investigate the chemical constituents of the n-butanol fraction of GCFS,to clarify the substance basis of efficacy.Methods The compounds were isolated and purified through D101 macroporous adsorbent resin,MCI-gel column chromatography,open ODS column chromatography and semi-preparative liquid chromatography.The structures of the compounds were identified according to physicochemical properties,hydrogen spectrum(^(1)H-NMR)and carbon spectrum(^(13)C-NMR)data.Results Six compounds were isolated from the n-butanol fraction of GCFS and identified as ginsenoside Rh21(1),ginsenoside Re_(3)(2),ginsenoside Rg_(1)(3),ginsenoside Re(4),ginsenoside Rc(5)and ginsenoside Rb_(1)(6).Conclusion All compounds were isolated from the GCFS for the first time.The compound 1 has not been reported in the original ginseng medicinal materials,which proves that the biotransformation technology can change the chemical composition of ginseng and provides a reference for the secondary development and utilization of ginseng medicinal materials.