异丙肾上腺素诱导的慢性应激焦虑样行为学检测

Detection of Chronic Stress Anxiety Behavior Induced by Isoproterenol

目的阐明异丙肾上腺素长期作用可以诱导昆明小鼠出现慢性应激焦虑样行为。方法50只昆明小鼠随机分为对照组、慢性应激组、异丙肾上腺素(Isoprenaline,ISO)组、慢性应激+ICI118,551(ICI)处理组、ISO+ICI 118,551(ISO+ICI)处理组,每组10只。对照组:每天腹腔注射生理盐水;慢性应激组:每天进行慢性应激处理;ISO处理组:每天腹腔注射ISO(5 mg/kg);慢性应激+ICI处理组:每天慢性应激处理后,腹腔注射ICI(0.2 mg/kg);ISO+ICI处理组:每天腹腔注射ISO(5 mg/kg)和ICI(0.2 mg/kg)。结果高架十字迷宫结果中,慢性应激组和ISO组开臂停留时间、进入开臂次数、开臂停留时间百分比和进入开臂次数百分比均显著低于对照组(P<0.05)。明暗箱结果中,慢性应激组和ISO处理组明箱停留时间、明箱停留时间百分比、明箱活动次数和穿梭次数显著低于对照组(P<0.05);暗箱停留时间显著高于对照组(P<0.05)。结论在β-AR激动剂异丙肾上腺素长期作用下,昆明小鼠出现了慢性应激焦虑样行为。

Objective To elucidate the long-term effect of isoproterenol on inducing anxiety-like behavior under chronic stress in kunming mice.Methods A total of 50 Kunming mice were randomly divided into control,chronic stress,isoproterenol(ISO)group,chronic stress+ICI 118,551(ICI)group and ISO+ICI 118,551(ISO+ICI)group(n=10 in each group).Control group:normal saline was intraperitoneally injected every day;Chronic stress group:Chronic stress treatment was given every day.ISO treatment group:ISO(5 mg/kg)was injected intraperitoneally every day.Chronic stress+ICI treatment group:ICI(0.2 mg/kg)was injected intraperitoneally every day after chronic stress treatment.ISO+ICI treatment group:ISO(5mg/kg)and ICI(0.2 mg/kg)were given intraperitoneally every day.Results The open arm residence time,the number of entering open arm,the percentage of open arm residence time and the number of entering open arm in the chronic stress group and ISO group were signifi cantly lower than those in the control group(P<0.05).The open-box retention time,percentage of open-box retention time,open-box activity times and shuttle times in the chronic stress group and ISO treatment group were signifi cantly lower than those in the control group(P<0.05).The retention time of dark box was signifi cantly higher than that of the control group(P<0.05).Conclusion After long-term treatment withβ-AR agonist isoproterenol,kunming mice developed anxiety-like behavior under chronic stress.