原肌球调节蛋白1与CDK1在机体水调节中的作用

The Role of Tropomyosin 1 and CDK1 in the Regulation of Body Water

目的分析原肌球调节蛋白1(tropomodulin 1,Tmod1)在机体水调节中的作用。方法采用小鼠肾脏原代内髓集合管细胞进行研究,通过使用腺病毒转染过表达/敲低Tmod1后,利用Western blot,实时荧光定量PCR,检测Tmod1蛋白及RNA水平的变化以验证腺病毒的效率;随后利用Western blot检测过表达/敲低Tmod1后细胞周期蛋白依赖性激酶1(cyclin dependent kinase 1,CDK1)蛋白表达变化。结果过表达Tmod1后,CDK1蛋白水平显著增加,当敲低Tmod1后CDK1蛋白表达下降,差异具有统计学意义(P<0.05)。结论Tmod1可能通过影响CDK1参与机体水调解的过程,但其机制有待深入研究。

Objective To analyze the role of tropomodulin 1(Tmod1)in the regulation of body water.Methods Using mouse kidney primary inner medullary collecting duct(IMCD)cells for the study,the primary cells were transfected with adenovirus to overexpress/knock down Tmod1.Western blot and real-time fl uorescent quantitative PCR were used to detect the changes in Tmod1 protein and RNA levels to verify adenovirus effi ciency.Subsequently,the expression of cyclin-dependent kinase 1 after overexpression/knockdown of TMOD1 was detected by Western blot.Results After the overexpression of Tmod1,the level of CDK1 protein increased signifi cantly,and decreased after knockdown of TMOD1,with statistical significance(P<0.05).Conclusion Tmod1 may participate in the process of body water mediation by infl uencing CDK1,but its mechanism remains to be further studied.