中性粒细胞蛋白-1、-2、-3在膀胱癌患者组织中的表达及与预后的相关性

Expression of neutrophil protein-1,neutrophil protein-2 and neutrophil protein-3 in bladder cancer and their correlation with prognosis

目的:研究中性粒细胞蛋白(HNP)-1、-2、-3在膀胱癌患者组织中的表达及与预后的关系。方法:采用免疫组织化学法检测膀胱癌组织中HNP-1、HNP-2、HNP-3的表达情况,分析HNP-1、HNP-2、HNP-3在膀胱癌组织中的表达与膀胱癌临床病理特征的相关性及预后相关因素的关系。结果:HNP-1、HNP-2、HNP-3蛋白在膀胱癌患者组织中的阳性表达率分别为70.51%、72.66%、71.94%,均明显高于癌旁正常组织8.10%、3.23%、6.45%,差异有统计学意义;HNP1、HNP2、HNP3在膀胱癌患者中的表达水平与肿瘤的病理分级、临床分期、淋巴结转移及复发等生物学行为相关;生存曲线结果提示这3种蛋白表达阳性的5年生存率均显著低于表达阴性的患者。Cox比例风险模型分析结果提示HNP-1、HNP-2、HNP-3的表达是影响膀胱癌预后的关键风险因素。结论:HNP-1、HNP-2、HNP-3在膀胱癌组织中高表达且表达程度随肿瘤发展进一步增加;HNP-1、HNP-2、HNP-3是影响患者预后的关键危险因素,阳性表达的患者5年生存率显著降低;HNP-1、HNP-2、HNP-3与膀胱癌病情发展、炎症程度及预后密切相关。

Objective:To study the expression of neutrophil protein-1(HNP-1),neutrophil protein-2(HNP-2)and neutrophil protein-3(HNP-3)in bladder cancer and their relationship with prognosis.Methods:The expression of HNP-1,HNP-2 and HNP-3 in bladder cancer was detected by immunohistochemical method,and the correlation between the expression of HNP-1,HNP-2 and HNP-3 and clinicopathological features and prognostic factors was analyzed.Results:The positive expression rates of HNP-1,HNP-2 and HNP-3 were 70.51%,72.66%and 71.94%,respectively in cancer tissues,which were significantly higher than those in normal tissues(8.10%,3.23%and 6.45%,respectively).The expression levels of HNP-1,HNP-2 and HNP-3 were significantly correlated with the pathological grade,clinical stage,lymph node metastasis and recurrence of bladder cancer.The 5-year survival rate of patients with positive expression of these three proteins was significantly lower than that of patients with negative expression.Cox proportional hazard model analysis showed that the expression of HNP-1,HNP-2 and HNP-3 was a key risk factor for the prognosis of bladder cancer.Conclusion:HNP-1,HNP-2 and HNP-3 are highly expressed in bladder cancer,and the degree of expression increases with tumor development.HNP-1,HNP-2 and HNP-3 are the key risk factors affecting the prognosis of patients,and the 5-year survival rate of patients with positive expression is significantly reduced.HNP-1,HNP-2,HNP-3 are closely related to the development,inflammation and prognosis of bladder cancer.