依达拉奉干预永久性脑缺血大鼠神经干细胞增殖及分化

Edaravone intervening the proliferation and differentiation of neural stem cells in rats with permanent cerebral ischemia

目的探讨依达拉奉对永久性脑缺血大鼠脑内内源性神经干细胞的影响。方法采用电凝法建立大鼠永久性脑缺血模型。将30只SD大鼠随机分为假手术组、脑损伤组与依达拉奉组,每组10只,模型制作成功后6 h,1.5 g/L依达拉奉组腹腔注射依达拉奉(10 ml/kg),每天1次,假手术组与脑缺血组腹腔注射等体积的生理盐水,连续注射7 d。末次给药后24 h,免疫荧光染色观察损伤侧脑室下区与缺血周围脑皮质区Brd U标记阳性细胞、Nestin/Brd U标记阳性细胞、神经元Ⅲ型β-微管蛋白(Tuj1)/Brd U标记阳性细胞与胶质纤维酸性蛋白(GFAP)/Brd U标记阳性细胞,Western blotting检测缺血周围脑皮质区Tuj1与GFAP蛋白表达。结果与脑缺血组比较,依达拉奉组损伤侧脑室下区与缺血周围脑皮质区的Brd U标记阳性细胞、Nestin/Brd U标记阳性细胞、Tuj1/Brd U标记阳性细胞与GFAP/Brd U标记阳性细胞明显增加(P<0.05)。与脑缺血组比较,依达拉奉组缺血周围脑皮质区的Tuj1与GFAP蛋白表达量增加(P<0.05)。结论依达拉奉可促进缺血损伤侧脑室下区与缺血周围脑皮质内源性神经干细胞与星形胶质细胞的增殖,促进内源性神经干细胞分化为神经元。

Objective To observe the effect of edaravone on endogenous neural stem cells in rats with permanent cerebral ischemia.Methods The rat model of permanent cerebral ischemia was established by electrocoagulation.Thirty SD rats were randomly divided into three groups: sham operation group,brain injury group and edaravone group.Six hours after the establishment of the model, the edaravone group was intraperitoneally injected with 1.5 g/L edaravone(10 ml/kg) once a day.The sham operation group and the cerebral ischemia group were intraperitoneally injected with saline of equal volume for 7 days.24 hours after the last administration,Brd U positive cells,Nestin/Brd U positive cells,neuronal class Ⅲ β-tubulin(Tuj1)/Brd U positive cells and glial fibrillary acidic protein(GFAP)/Brd U positive cells were observed by immunofluorescent staining,Tuj1 and GFAP protein expressions were detected by Western blotting.Results Compared with the cerebral ischemia group,the Brd U positive cells,Nestin/Brd U positive cells,Tuj1/Brd U positive cells and GFAP/Brd U positive cells increased significantly in the infraventricular area and the cortex area around the ischemia in the edaravone group(P<0.05).Compared with the cerebral ischemia group,the expression of Tuj1 and GFAP protein in the cerebral cortex of edaravone group increased(P < 0.05).Conclusion Edaravone can promote the proliferation of endogenous neural stem cells and astrocytes in the subventricular area and the cortex around ischemia,and promote the differentiation of endogenous neural stem cells into neurons.