结直肠癌内脂素、hsa-miR-199a、细胞外信号调节蛋白激酶1/2的表达及临床意义

Expression and clinical significance of visfatin,hsa-miR-199a and extracellular signal regulating protein kinase 1/2 in colorectal cancer

目的探讨结直肠癌(CRC)组织中内脂素(visfatin)、hsa-miR-199a(miR-199)和细胞外信号调节蛋白激酶1/2(ERK1/2)的表达及三者的相关性。方法 Real-time PCR检测2017年1月~2017年12月期间在齐齐哈尔医学院临床病理诊断中心进行临床病理检验的根治性切除原发结直肠癌64例visfatin mRNA和hsa-miR-199a的表达水平,免疫组织化学法和Western blotting检测visfatin和ERK1/2蛋白表达水平,按visfatin和ERK1/2蛋白表达程度分组,比较各组hsa-miR-199a相对表达量。结果结直肠癌组织(观察组,64例)中visfatin基因水平和蛋白水平均高于正常结直肠组织(对照组),表达具有一致性;结直肠癌组织(观察组,64例)中hsa-miR-199a的相对表达量低于正常结直肠组织(对照组)与visfatin mRNA成显著负相关;ERK1/2的结直肠癌组织(观察组)表达高于正常结直肠组织(对照组)与visfatin成显著正相关。Visfatin、ERK1/2蛋白表达阳性组,hsa-miR-199a相对表达量与检验变量比较差异具有显著性(P<0.05)。结论结直肠癌visfatin过表达影响hsa-miR-199a的下调且与ERK信号通路有关。

Objective To investigate the expression and correlation of visfatin,hsa-miR-199a and extracellular regulated protein kinase 1/2(ERK1/2) in colorectal cancer(CRC) tissues.Methods The expression levels of visfatin mRNA and hsa-miR-199a were detected by Real-time PCR in 64 primary cases of radical resection of colorectal cancer who underwent pathological clinical examination in the clinicopathological examination in our hospital center from January 2017 to December 2017,the protein expression levels of visfatin and ERK1/2 were detected by immunohistochemistry and Western blotting.The relative expression levels of hsa-miR-199a in each group were compared according to the expression levels of visfatin and ERK1/2.Results The levels of visfatin gene and protein in colorectal cancer tissue(observation group,64 cases) were higher than those in normal colorectal cancer(control group,64 cases),and the expression was consistent.The colorectal cancer tissue(observation group) expression level of hsa-miR-199a was lower than that of normal colorectal tissue(control group),and visfatin mRNA was significantly negatively correlated.The colorectal cancer tissue(observation group) expression of ERK1/2 was higher than normal colorectal tissue(control group) and visfatin was significantly positively correlated.The relative expression of hsa-miR-199a in the visfatin and ERK1/2 positive group was significantly different from that in the test variables.Conclusion The overexpression of visfatin in colorectal cancer affects the down-regulation of hsa-miR-199a and is related to the ERK signaling pathway.