硬脂酸单甘油酯协同卡泊芬净的体外抗念珠菌活性研究

Study on the synergistic antifungal activity of glyceryl monostearate and caspofungin in vitro

目的通过化合物体外筛选,获得与临床常用抗真菌药物卡泊芬净具有协同作用的化合物,增强卡泊芬净的抗菌效果。方法采用棋盘式微量液基稀释法考察硬脂酸单甘油酯(glyceryl monostearate,GMS)与卡泊芬净(CAS)、氟康唑(FCZ)对多种念珠菌的最低抑菌浓度(MIC)和协同指数(FICI);考察硬脂酸单甘油酯与卡泊芬净单用及联合使用时,在RPMI 1640和Spider培养基中抑制白念珠菌菌丝形成的抑菌浓度。结果在考察的绝大多数致病念珠菌中,硬脂酸单甘油酯与卡泊芬净具有协同抗菌作用,协同指数FICI≤0.5;联用硬脂酸单甘油酯后,卡泊芬净抑制菌丝形成的浓度降低。结论硬脂酸单甘油酯可以增强卡泊芬净的体外抗真菌效果,二者具有较强的协同抗念珠菌活性。

Objective In order to improve the antifungal effect of caspofungin,we screened the synergistic effects of many compounds with caspofungin in vitro.Methods The minimum inhibitory concentration(MIC)and synergistic index(FICI)of glyceryl monostearate(GMS),caspofungin(CAS)and fluconazole(FCZ)against Candida were measured by a microdilution method.In addition,the minimum concentration of glyceryl monostearate and caspofungin used alone or in combination to inhibit the hyphae formation of Candida albicans was investigated in the RPMI 1640 and Spider medium.Results The combination of glyceryl monostearate and caspofungin showed synergistic antifungal effect on pathogenic Candida,and the synergistic index was less than 0.5.When combined with glyceryl monostearate,the concentration of caspofungin inhibiting the formation of hyphae decreased significantly.Conclusion Glyceryl monostearate could enhance the antifungal effect of caspofungin in vitro.