响应面法优化三氯化铁回收河鲀鱼糜漂洗液中蛋白质的研究

Optimization of the recovery of protein from washing solution of the Fugu surimi by FeCl_(3) flocculation

漂洗是鱼糜加工中重要的步骤之一。鱼肉在漂洗过程中会损失大量水溶性蛋白质。对鱼糜漂洗液进行蛋白质回收,可以提高鱼糜利用率,降低污水处理成本,减少环境污染。本试验采用絮凝剂三氯化铁(FeCl_(3))对河鲀鱼糜漂洗液中的水溶性蛋白质进行回收,根据不同的单因素变量,研究FeCl_(3)絮凝法回收鱼糜漂洗液中蛋白质的最佳条件。通过响应面分析法对试验条件进行优化,寻求最佳的组合条件。结果显示,FeCl_(3)法回收河鲀鱼糜漂洗液中水溶性蛋白质的最佳条件为pH 6、温度65℃、每10 mL鱼糜漂洗液添加1%1.4 mL FeCl_(3)溶液。

Rinse is one of the important steps of minced fish processing.Lots of soluble protein would be lost in the process of rinsing.So,recovering protein from the minced fish rinse solution can improve the utilization rate of the surimi,reduce the cost of sewage treatment steps,and decrease the pollution of environment.In this paper,FeCl_(3) as a flocculant was used to retrieve the water soluble protein from the surimi rinse water.According to different single factor variables,the optimal conditions of the recovery of protein from the surimi rinse water by FeCl_(3) flocculation method were researched and optimized by response surface analysis.Results showed that the optimal conditions of FeCl_(3) recovery proteins were:pH 6,temperature of 65℃,and an addition amount of 1%FeCl_(3) solution of 1.4 mL in 10 mL minced fish rinse solution.