红毛藻多糖的提取工艺优化及其抗氧化活性

Optimization of extraction conditions and investigation of the antioxidant activity of polysaccharides from Bangia fusco-purpurea

本文以红毛藻为研究材料,对红毛藻多糖的提取工艺进行优化,并对其体外抗氧化活性进行研究。实验分别考察红毛藻的提取时间、提取温度、料液比对红毛藻多糖提取量的影响,并结合正交实验设计优选出最佳提取工艺,同时通过体外抗氧化活性评价研究其清除DPPH自由基和羟基自由基的能力。结果表明,以料液比1∶40(m/v)、在90℃下提取2.0 h为最佳提取工艺,在此工艺下红毛藻多糖提取量最高,达到(77.57±2.17)mg/g;体外抗氧化活性评价结果显示,红毛藻多糖对DPPH自由基和羟基自由基具有明显的清除效果,清除DPPH自由基的IC 50值为0.36 mg/mL,清除羟基自由基的IC 50值为0.65 mg/mL。本研究可为红毛藻多糖的进一步开发应用提供参考。

In the present study,the extraction process of polysaccharide from Bangia fusco-purpurea was optimized and the antioxidant activity of polysaccharide was studied in vitro.The effects of the extraction time,extraction temperature and ratio of material to liquid on the extraction amount of polysaccharide from B.fusco-purpurea were investigated,respectively,and the optimum preparation conditions were selected with orthogonal design combined with multi-index test.Meanwhile,the antioxidant activity of polysaccharide from B.fusco-purpurea was evaluated by DPPH free radical and hydroxyl radical scavenging method.The results showed that the optimum extraction conditions were the ratio of solid to liquid was 1∶40(m/v)at 90℃for 2.0 h.Under the optimum extraction conditions,the maximum extraction yield of polysaccharide reached(77.57±2.17)mg/g.Additionally,the result of antioxidant activity in vitro showed that the polysaccharide form B.fusco-purpurea presented significant scavenging effects on DPPH free radical and hydroxyl free radical.The IC 50 value of DPPH free radicals scavenging was 0.36 mg/mL,and the IC 50 value of hydroxyl free radicals scavenging was 0.65 mg/mL.This study can be used for the further development and application of B.fusco-purpurea polysaccharide.