基于高通量测序技术分析青岛市典型海滩沉积物的微生物多样性

Analysis of microbial community of typical beach sediments in Qingdao city using high throughput sequencing

为了解青岛典型海滩沉积物环境中微生物群落结构的组成及差异性,采集了青岛市五大典型海水浴场的近海沉积物样品,利用高通量测序技术研究了不同沉积物环境中微生物群落结构的组成差异。结果表明,在门的水平上,五大海水浴场沉积物中变形菌门(Proteobacteria)占主导地位。主坐标分析(principal co-ordinates analysis,PCoA)的结果表明,五大海水浴场沉积物环境中的微生物群落结构组成存在明显差异(p<0.05)。通过比对致病菌数据库发现,五大海水浴场沉积物环境中检测到潜在致病菌属13种,包含致病菌种共有17种。其中,弧菌属(Vibrio)的相对丰度均高于其他潜在致病菌属,且在第三和金沙滩海水浴场中相对丰度最高,对海洋环境生态及海产品养殖造成一定的影响。

In order to understand the composition and difference of microbial community structure in the environment of typical beach sediments in Qingdao, sediments in five classical beaches of Qingdao have been collected and the microbial community structure has been studied using high-throughput sequencing technique.The results showed that Proteobacteria were dominant in the sediment of five beaches. The PCoA analysis indicated that the microbial community composition was significantly different(p<0.05) between five sediments. By comparing with the pathogenic bacterial database, 13 genera of potential pathogenic bacteria were detected in the sediment environment of the five beaches, including 17 species of pathogenic bacteria.Among them, the relative abundance of vibrio is higher than that of other potential pathogenic bacteria, and the relative abundance is the highest in the third and golden beaches, which may cause certain environmental risks to the marine environmental ecology and marine farming.