Myosin1H基因多态性与蒙古族骨性下颌后缩易感性的关系

The Correlation Between Myosin1H Gene Polymorphism and the Susceptibility of Skeletal Mandibular Retrusion in Mongolian

目的:分析I类肌球蛋白(Myosin1H)基因多态性与蒙古族骨性下颌后缩(MR)易感性的关系。方法:纳入我院口腔科就诊的以MR为主的骨性Ⅱ类错合畸形61例作为观察组,同期就诊骨性Ⅰ类错合畸形61例作为对照组,均为内蒙古地区蒙古族病例,聚合酶链式反应-限制性片段长度多态性(PCR-RELP)法测定Myosin1H基因rs11611277、rs3825393位点基因多态性分布,分析Myosin1H基因多态性与蒙古族骨性MR易感性关系。结果:观察组携带AG基因型(60.66%)及A等位基因频率(38.52%)高于对照组(24.59%、23.77%)(P<0.05);观察组携带rs3825393位点AG基因型患者SNB角[(81.49±1.73)度]、Z角[(62.25±4.75)度]低于携带GG型[(82.18±1.57)度、(82.16±1.95)度]与AA型[(72.14±5.11)度、(73.52±6.34)度]患者(P<0.05),ANB角[(5.91±0.75)度]高于携带GG型[(4.21±1.45)度]、AA型患者[(4.19±0.65)度](P<0.05);以GG型作为参照组,携带Myosin1H基因rs3825393位点AG基因型可增加MR易感性2.571倍(P<0.05)。结论:Myosin1H基因rs3825393位点G→A突变与蒙古族MR易感性有关,携带该位点AG基因型患者MR易感风险更大。

Objective:To analyze the correlation between polymorphism of type I myosin(myosin1H)gene and the susceptibility of skeletal mandibular retrusion(MR)in Mongolian.Methods:A total of 61 patients with skeletal type II malocclusion(mainly on MR)who were treated in stomatology department of the hospital were made the observation group,while another 61 patients with skeletal type I malocclusion were made the control group.All were Mongolian cases in Inner Mongolia.The distribution of Myosin1H gene polymorphisms at rs11611277 and rs3825393 loci was detected by polymerase chain reaction-restriction fragments length polymorphism(PCR-RELP).The correlation between Myosin1H gene polymorphism and the susceptibility of skeletal MR was analyzed.Results:The genotype carrying AG and frequency of A allele in observation group were 60.66%and 38.52%,higher than those in control group(24.59%,23.77%)(P<0.05).In observation group,SNB angle and Z angle in patients carrying AG genotype at rs3825393 locus wee(81.49±1.73)°and(62.25±4.75)°,lower than those carrying GG genotype[(82.18±1.57)°,(82.16±1.95)°]and AA genotype[(72.14±5.11)°,(73.52±6.34)°](P<0.05),while ANB angle[(5.91±0.75)°]was higher than those carrying GG genotype[(4.21±1.45)°]and AA genotype[(4.19±0.65)°](P<0.05).Taking GG genotype as control group,carrying AG genotype of Myosin1H gene at rs3825393 could increase the susceptibility to MR by 2.571 times(P<0.05).Conclusion:G→A mutation of Myosin1H gene at rs3825393 locus is related to the susceptibility of MR in Mongolian.The risk of susceptibility to MR is higher in patients with AG genotype at the locus.