T细胞免疫球蛋白与黏蛋白结构域蛋白-3对2型糖尿病患者T细胞的影响

Effects of T Cell Immunoglobulin and Mucin Domain Protein-3 on T Cells in Patients with Type 2 Diabetes

目的:探讨T细胞免疫球蛋白与黏蛋白结构域蛋白-3对2型糖尿病患者T细胞的影响。方法:本研究选取2017年1月至2019年12月在本院的内分泌科就诊的72例2型糖尿病患者作为糖尿病组,并选取同期进行健康体检者55例作为对照组;比较两组患者T细胞中TIM-3的表达情况,比较两组患者T细胞分泌细胞因子相对量。进一步比较通过抗TIM-3抗体刺激对糖尿病患者T细胞分泌细胞因子相对量。结果:糖尿病组患者的CD4+T和CD8+T细胞的含量均显著高于对照组(P<0.05);糖尿病组患者的INF-γ、IL-4、IL-17、IL-22含量低于对照组,糖尿病组患者的IL-10、IL-35含量高于对照组(P<0.05);糖尿病组TNF-α、INF-γ的含量低于对照组(P<0.05);经过抗TIM-3抗体刺激后的糖尿病患者INF-γ、IL-17、IL-22的含量明显高于无抗体刺激糖尿病患者,经过抗TIM-3抗体刺激后糖尿病患者IL-35的含量明显低于无抗体刺激糖尿病患者(P<0.05);经过抗TIM-3抗体刺激后的糖尿病患者TNF-α、INF-γ含量高于无抗体刺激糖尿病患者(P<0.05)。结论:在糖尿病患者中T细胞表面黏蛋白结构域蛋白-3的表达量提升,含量会升高,其可能参与了2型糖尿病患者T细胞调节功能。

Objective:To investigate the effect of T immunoglobulin and mucin domain protein-3 on T cells in type 2 diabetes mellitus.Methods:This study selected 72 patients with type 2 diabetes from January 2017 to December 2019 and 55 healthy controls as the control group to compare the expression of TIM-3 in T cells.The relative expression of cytokines was compared between the two groups.To further compare the relative expression of cytokines and transcription factors stimulated by anti TIM-3 antibody on T cells of diabetic patients.Results:The contents of CD4+T and CD8+T cells in diabetic group were significantly higher than those in control group(P<0.05);diabetic group had lower INF-γ、IL-4、IL-17、IL-22 content than control group.The IL-10、IL-35 content of diabetic group was higher than that of control group(P<0.05).The content of TNF-α、INF-γin diabetic group was lower than that in control group(P<0.05).The INF-γ、IL-17、IL-22 content of diabetic patients stimulated by anti-antibody TIM-3 was significantly higher than that of non-antibody stimulated diabetic patients.The IL-35 content of diabetic patients after anti-antibody stimulation was significantly lower than that of non-antibody stimulation diabetic patients(P<0.05).The TNF-α、INF-γcontent of diabetic patients after anti-antibody stimulation was higher than that of non-antibody stimulation diabetic patients(P<0.05).Conclusion:The expression of T cell surface mucin domain protein-3 is increased in diabetic patients.The content will rise,and may be involved in the cellular regulatory function T type 2 diabetes patients.