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瘦素对肥胖饮食小鼠的脂质合成相关基因表达影响的研究 被引量:2

Effects of leptin on the expression of lipid synthesis related genes in obese diet mice
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摘要 目的探讨瘦素对肥胖小鼠脂质合成相关基因表达的影响。方法通过高脂膳食喂养小鼠构建肥胖小鼠模型,并将肥胖小鼠随机分为四组:高脂肪组、瘦素低剂量组(0.5mg/kg)、瘦素高剂量组(1.0 mg/kg)、正常对照组(正常膳食喂养小鼠),每组4只。连续喂养4周后,采用Realtime PCR检测各组大鼠肝脏组织固醇调节元件结合蛋白-1(SREBP-1)、脂肪酸合酶(FAS)、乙酰辅酶A羧化酶(ACC-1)及过氧化物酶体增殖物激活受体γ(PPARγ)mRNA表达。结果与正常组比较,高脂肪组小鼠肝脏组织中PPARγ、ACC-1、FAS、SREBP1 mRNA表达量均显著下调(P<0.01);与高脂肪组比较,瘦素低、高剂量组小鼠肝脏组织中PPARγmRNA表达量上调,瘦素高剂量组中小鼠肝脏组织中ACC-1、FAS、SREBF1 mRNA表达量显著上调(P<0.01)。结论瘦素通过调节脂质合成相关基因表达影响肥胖饮食小鼠脂质代谢。 Objective To explore effects of leptin on the expression of lipid synthesis related genes in obese diet mice.Methods Obese mice model was established by feeding with high fat diet.The obese mice were randomly divided into high-fat group,low and high-dose leptin group(0.5,1.0 mg/kg).In addition,the normal diet fed mice were set as the normal control group,with 4 mice in each group.After 4 weeks of continuous feeding,the mRNA expressions of sterol regulatory element binding protein-1(SREBP-1),Fatty acid synthase(FAS),Acetyl-Coa carboxylase 1(ACC1)and Peroxisome proliferator-activated receptor gamma(PPARγ)were detected by realtime PCR.Results Compared with the normal group,the expression PPARγ,ACC-1,FAS and SREBP1 mRNA in liver tissue of mice in high-fat group was significantly decreased(P<0.01).Compared with the high-fat group,the expression of PPARγmRNA in liver tissue of mice in low-dose and high-dose leptin group was increased,the expression of ACC-1,FAS and SREBP1 mRNA in liver tissue of mice in high-dose leptin group was significantly increased(P<0.01).Conclusion Leptin could affect lipid metabolism in obese diet mice by regulating the expression of lipid synthesis related genes.
作者 石卫红 李小林 熊清华 万冠群 曾琪 胡云刚 覃煦 SHI Wei-hong;LI Xiao-lin;XIONG Qing-hua;WAN Guan-qun;ZENG Qi;HU Yun-gang;QIN Xu(Plastic surgery,Jiangxi Province People's hospital,Jiangxi Province,330006,China)
出处 《中国医疗美容》 2021年第5期48-51,共4页 China Medical Cosmetology
关键词 瘦素 肥胖 脂质合成 基因表达 leptin obese lipid synthesis gene expression
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