小鼠脓毒症进程中IL-6的动态变化及其前期主要脏器损伤

IL-6 dynamic changes during the sepsis development and main organs damages in the early stage of sepsis in mice

目的探讨小鼠脓毒症进程中白细胞介素-6(IL-6)的动态变化及其前期主要脏器损伤。方法取7~8周C57雄性小鼠30只,按照数字表法随机分为对照组和脓毒症0、4、8、12、16 h组,每组5只。脓毒症各组小鼠首先用脂多糖(LPS)按照400μg/kg进行预处理8 h,然后继续使用LPS 10 mg/kg的剂量刺激小鼠,建立脓毒症小鼠模型。对照组仅给予1ml/kg的生理盐水尾静脉注射,不作其他处理。采用酶联免疫吸附(ELISA)法测定小鼠血清IL-6含量,并观察其浓度变化;同时取脓毒症模型小鼠肝、肾、心、肺、脾等脏器进行病理学组织切片,探究其发展进程中的病理学变化。结果0、4、8、12、16 h组脓毒症小鼠血清IL-6水平分别为[(239.7±21.5)、(1268.5±48.7)、(1836.8±67.4)、(2393.9±68.3)、(2989.5±84.7)]pg/ml,与对照组[(56.5±1.8)pg/ml]相比差异均有统计学意义(P<0.01);病理检查结果显示,在脓毒症早期,最先受损的脏器为肝脏、心脏,而肾脏、肺、脾脏影响较小。结论模型小鼠脓毒症病情越重,血清IL-6水平越高,且脏器中心脏、肝脏最先出现损伤。

Objective To investigate the dynamic changes of IL-6 during the sepsis development and the main organ damages in the early stage of sepsis in mice.Methods A total of 30 C57 male mice aged 7-8 weeks were randomly divided into control group and sepsis 0,4,8,12,16 h groups with 5 mice in each group.The mice in each sepsis group were pretreated by lipopolysaccharide(LPS)with 400μg/kg for 8 hours,and then stimulated by LPS with 10 mg/kg to establish sepsis models.The control group was only given saline with 1 ml/kg by tail vein injection without any other treatment.Enzyme linked immunosorbent assay(ELISA)was used to measure the serum IL-6 content and observe its concentration changes;meanwhile,the liver,kidney,heart,lung,spleen,and other organs of sepsis model mice were taken for pathological tissue sections to explore the pathological changes in the sepsis development process.Results The levels of serum IL-6 were(239.7±21.5),(1268.5±48.7),(1836.8±67.4),(2393.9±68.3),(2989.5±84.7)pg/ml in the sepsis 0,4,8,12,16 h groups,which were significantly higher than those in the control group(56.5±1.8)pg/ml.The results of pathological examination showed that in the early stage of sepsis,the organ damages firstly occurred in heart and liver,while the kidney,lung,and spleen were less affected.Conclusion As the sepsis in model mice become severe,the serum IL-6 level increases and the heart and liver firstly show organ damages among all the main organs.