芹黄素对脑缺血再灌注损伤保护作用的实验研究

Protective effect of apigenin against cerebral ischemia/reperfusion injury

目的:观察芹黄素对脑缺血再灌注损伤沙土鼠脑保护作用及炎症反应的影响。方法:采用双侧颈总动脉夹闭手术法建立脑缺血再灌注损伤沙土鼠模型,将其随机分为模型组、尼莫地平组(2 mg/kg)、芹黄素组(40 mg/kg)、假手术组,建模前1周开始腹腔注射给药,再灌注6 h内记录各组神经症状并做卒中评分,再灌注6 h后以免疫组化法测定脑组织海马CA1区核转录因子-κB(NF-κB)的表达。结果:芹黄素组和尼莫地平组脑缺血再灌注损伤沙土鼠的卒中评分、脑部海马CA1区NF-κB,与模型组相比,差异有统计学意义(P<0.01);与假手术组脑部极低的NF-κB表达相比,模型组沙土鼠海马CA1区NF-κB表达明显升高(P<0.01)。结论:芹黄素可抑制核转录因子的激活,调控炎症因子表达,减轻脑部炎症反应,实现对脑缺血再灌注损伤沙土鼠的脑保护作用。

Objective: To investigate the cerebral protection effect of apigeninagainst cerebral ischemia/reperfusion injury in gerbils and its effect on inflammatory response.Methods: Clipping operation of both common carotid arteries was used to establish a model of cerebral ischemia/reperfusion injury in gerbils, and then the gerbils were randomly divided into model group, nimodipine group(2 mg/kg),apigenin group(40 mg/kg),and sham-operation group.The drugs were intraperitoneally injectedsince1 week before modeling.Neurological symptoms and stroke score wererecorded within 6 hours of reperfusion, and after 6 hours of reperfusion, immunohistochemistry was used to measure the expression of nuclear factor-kappa B(NF-κB) in the hippocampal CA1 region.Results: There were significant differences in stroke score and the expression of NF-κB in the hippocampal CA1 region between the apigenin group and the model group and between the nimodipine group and the model group(P<0.01).Compared with the sham-operation group with an extremely low expression level of NF-κB in brain, the model group had a significant increase in the expression of NF-κB in the hippocampal CA1 region(P<0.01).Conclusion: Apigenin can inhibited the activation of NF-κB,regulate the expression of inflammatory factors, alleviate inflammatory response in brain, and thus exert a cerebral protection effect in gerbils with cerebral ischemia/reperfusion injury.