植物赤霉素受体GID1基因的分析

Analysis of Gibberellin Receptor GID1 Gene in Plants

从NCBI数据库在线网站搜集了赤霉素受体基因核苷酸序列,分析了所获基因的进化关系。结果表明,植物的98个GID1基因拥有共同的根系,进化为Group A、Group B、Group C和Group D 4组。Group A GID1基因在单子叶植物纲中没有GID1a、GID1b、GID1c分类现象,在双子叶植物纲中基因有简单的Group B GID1b,Group C GID1a,且GID1c聚类现象;Group D由被子植物门单子叶植物和双子叶植物及苔藓植物门小立苔藓植物的基因组成;GID1基因组在进化过程中可能出现复制突变,所有GID1基因具有相同的保守区域,可利用基因之间的保守性来克隆未知基因;102条序列归属于91种植物,尚有更多单子叶植物、裸子植物和蕨类植物的GID1基因有待克隆。

The nucleotide sequence of gibberellin receptor gene was collected from the NCBI database online website,and the evolutionary relationship of the obtained gene was analyzed.The results showed that 98 GID1 genes in plants had the same root system,which evolved into four groups:Group A,Group B,Group C and Group D.Group A GID1 gene has no classification phenomenon of GID1 a,GID1 b and GID1 c in monocotyledons.In dicotyledons,there are simple Group B GID1 b,Group C GID1 a and GID1 c clustering phenomenon.Group D is composed of GID1 genes of Monocotyledoneae and Dicotyledoneae of the Angiospermae and Bryophyta Bryopsida of the plants.This suggests that the GID1 genome is replicating during the evolutionary process.All GID1 genes share the same conserved region,which can be used to clone unknown genes.Phylogenetic analysis of 102 GID1 genes of 91 plant species shows that more GID1 genes of monocotyledons,gymnosperms and ferns need to be cloned.