超声辅助萃取六味地黄丸中没食子酸工艺优化

Optimization of Ultrasonic-Assisted Extraction Process of Gallic Acid from Liuwei Dihuang Pills

采用超声辅助萃取法从六味地黄丸中提取没食子酸,考察溶剂比例(甲醇∶0.1%乙酸)、超声温度、超声时间和超声功率对六味地黄丸中没食子酸提取率的影响,通过单因素试验和正交试验研究六味地黄丸中没食子酸超声辅助萃取法的最佳工艺条件。结果表明,六味地黄丸中没食子酸的最佳工艺条件为溶剂比例(甲醇∶0.1%乙酸)2∶8、超声温度40℃、超声时间30 min、超声功率180 W。没食子酸在1.6~19.2μg/mL线性关系良好(R^(2)=0.999),平均加标回收率为99.3%,相对标准偏差(RSD)为2.5%(n=3)。超声辅助萃取法与浸渍提取法相比,提取没食子酸含量增加了17.14%~23.62%。

Ultrasound-assisted extraction method was used to extract gallic acid from Liuwei Dihuang Pills,and the effects of solvent ratio(methanol∶0.1%acetic acid),ultrasonic temperature,ultrasonic time and ultrasonic power on the extraction rate of gallic acid in Liuwei Dihuang Pills were investigated.The optimal technological conditions of gallic acid in Liuwei Dihuang Pills were studied by single factor experiment and orthogonal experiment.The results showed that the optimal process conditions for the ultrasonic-assisted extraction of gallic acid in Liuwei Dihuang Pills were as follows:solvent ratio 2∶8,ultrasonic temperature 40℃,ultrasonic time 30 min and the ultrasonic power 180 W.Gallic acid had a good linear relationship in the range of 1.6-19.2μg/mL(R^(2)=0.999),the average recovery rate was 99.3%,and the relative standard deviation(RSD)was 2.5%(n=3).Compared with the impregnation method,the content of gallic acid extracted by ultrasonic assisted extraction increased by 17.14%-23.62%.