茄子脂氧合酶家族基因全基因组鉴定与表达分析

Genome-wide identification and expression analysis of LOX gene family in eggplant(Solanum melongena)

脂氧合酶(lipoxygenase,LOX)是一类重要的含非血红素铁的蛋白,在植物生长发育、响应生物和非生物胁迫时起重要作用。为进一步明确茄子中LOX基因及其进化关系,对茄子全基因组进行分析筛选,共鉴定出12个SmeLOX基因。它们分布在茄子1、3、8和9号染色体上。系统发育树分析表明,茄子LOX家族基因可分为9-LOX、type I 13-LOX和type II 13-LOX 3类。此外,利用实时荧光定量PCR对鉴定出的12个茄子SmeLOX基因进行组织表达分析,结果表明,SmeLOX1、SmeLOX4、SmeLOX6和SmeLOX8基因在茄子5种组织(根、茎秆、叶片、花、果实)中均有表达。研究结果为进一步研究SmeLOX基因在茄子生长发育过程中的功能提供了科学依据。

Lipoxygenase(LOX)is a class of non-heme iron-containing dioxygenase which plays important role in plant growth and biotic/abiotic stress tolerance.To explore LOX family genes and their evolutionary relationship in eggplants,twelve candidates,which were distributed on chromosomes 1,3,8,and 9,were identified as SmeLOX gene by genome-wide analysis.Phylogenetic analysis showed that SmeLOX genes were classified into three categories(9-LOX,type I 13-LOX,and type II 13-LOX).In addition,real-time quantitative PCR analysis demonstrated that only the expression of SmeLOX1,SmeLOX4,SmeLOX6,and SmeLOX8 were detected in all tissues of eggplant,including roots,stems,leaves,flowers and fruits.Overall,this study provided scientific basis for further function analysis of the SmeLOX genes during growth and development of eggplant.