双标线性校正法对茵栀黄胶囊液相色谱峰的定性分析

Qualitative analysis of chromatographic peaks based on liner calibration with two reference substances-taking Yinzhihuang capsules as an example

目的:采用高效液相色谱法对茵栀黄胶囊中绿原酸、新绿原酸、隐绿原酸、栀子苷、对羟基苯乙酮进行分析,使用双标线性校正法进行保留时间预测,考察其用于色谱峰定性的可行性。方法:测定茵栀黄胶囊中5个成分在16根不同色谱柱上的实际保留时间。经筛选,双标化合物选定为峰1新绿原酸和峰5对羟基苯乙酮,采用双标线性校正法对保留时间进行预测,并在其他4根色谱柱上进行验证。同时选择中间峰绿原酸为参照物,采用相对保留时间法对保留时间进行预测。对这2种方法的优劣进行分析。结果:经比较,双标线性校正法保留时间预测正确率更高(96.36%),且有更高的色谱柱适用性(11根色谱柱)。结论:双标线性校正法可以用于色谱峰的辅助定性,是1种新的替代对照品方法。

Objective:To analyze chlorogenic acid,neo-chlorogenic acid,crypto-chlorogenic acid,geniposide and p-hydroxyacetophenone in Yinzhihuang capsules by HPLC,to predict the retention time using dualstandard linear correction method,and to investigate the feasibility of the method in qualitative analysis of chromatographic peaks.Methods:The actual retention time of 5 components in Yinzhihuang capsules on 16 different chromatographic columns were determined.After screening,the dual-label compounds were selected as neo-chlorogenic acid and p-hydroxyacetophenone,and the dual-standard linear correction method was used to predict the retention time and the method was verified on the other 4 chromatographic columns.At the same time,chlorogenic acid was selected as the reference substance,and the relative retention time method was used to predict and analyze the retention time.Results:After comparison,the dual-standard linear calibration method had higher retention time prediction accuracy(96.36%)and higher column adaptability(11 columns).Conclusion:The dual-standard linear correction method can be used for qualitative analysis and can be used as a new alternative reference method.