不同水质水体中溶藻微生物种群多样性研究

Algae-lysing Microbial Community Analysis of Natural Different Quality Water

以不同水质水体为研究对象,采用SDS-PAGE电泳,结合LC-MS质谱分析,对溶藻蛋白酶进行了初步鉴定与分析研究;并结合PCR-DGGE技术对16S r DNA进行鉴定,通过对目的条带的克隆、测序,结果与NCBI数据库比对,利用MEGA软件绘制进化树对细菌的同源性进行分析,从而确定自然水体中微生物菌群结构,并分析加藻前后菌群的变化规律。综合分析蛋白质鉴定结果和测序比对结果,发现两者能够较好地吻合,如Klebsiella,Acinetobacter,Enterobacter,Bacillus和Pseudomonas等在2种鉴定结果中均有出现,其中Klebsiella,Acinetobacter,Enterobacter,Bacillus和Pseudomonas在有关研究中被认为具有溶藻作用。

Took the different quality waters as the research object, Algae-lysing protease was preliminarily identified and analyzed by SDS-PAGE and LC-MS, with the PCR-DGGE being used to analyze the microorganisms in waters, and the target bands were cloned and sequenced for identifying the population of 16 S r DNA. These sequences were compared with NCBI, and constructed the phylogenetic tree and homology analysis of the bacteria by using the MEGA software.With the microbial community structure and its change law in natural waters determined. The result of comprehensive analysis of protein and gene sequence revealed obvious correlativity between them, such as Klebsiella,Acinetobacter,Enterobacter,Bacillus and Pseudomonas and so on were all found in these two results, among them Klebsiella,Acinetobacter,Enterobacter,Bacillus and Pseudomonas were considered to have the ability to kill algae.