低温胁迫下4份烟草品种苗期叶绿素及渗透调节物质含量的变化

Changes of Chlorophyll and Osmotic Adjustment Substances in 4 Tobacco Cultivars at Seedling Stage under Low Temperature Stress

试验以4份烟草品种为材料,对7~8叶龄的幼苗进行低温处理,研究低温胁迫进程中干物重、叶绿素和渗透调节物质等生理指标的变化规律,且利用隶属函数法对不同烟草品种耐寒性进行综合排序。结果表明:低温胁迫期间,低温处理的干物质和叶绿素积累量均小于对照组,其降幅由大到小依次为大兰花、NC89、延晒9号和龙江981;4份烟草品种的可溶性糖与脯氨酸含量随低温胁迫时间的延长均逐渐增加,而可溶性蛋白含量均逐渐下降;其中,可溶性糖和脯氨酸增幅最大的均为龙江981;可溶性蛋白含量降幅最大的为大兰花。基于隶属函数的耐寒性综合排序由强到弱依次为龙江981、延晒9号、NC89和大兰花。

In this experiment,4 tobacco varieties were used as materials,and 7~8 leaf-age seedlings were treated with low temperature to study the changes of dry matter weight,chlorophyll and osmotic adjustment substances during the process of low temperature stress.And,the membership function method was used for sorting different tobacco varieties comprehensively by their cold resistances.The results showed that during the low temperature stress,the dry matter and chlorophyll accumulation of the low temperature treatment were less than those of the control,and the decreasing amplitudes were Dalanhua,NC89,Yanshai No.9 and Longjiang 981 in descending order,compared with that of the control.The soluble sugar and proline contents of 4 tobacco varieties gradually increased with the extension of low temperature stress time,while the content of soluble protein gradually decreased.Among them,the largest increase of soluble sugar and proline was Longjiang 981;the largest decrease of soluble protein content was the large orchid.The comprehensive ranking of cold tolerance based on membership function from strong to weak was Longjiang 981,Yanshai No.9,NC89 and Dalanhua.