登革病毒模拟ADE效应中肝细胞lncRNA的差异表达

Differential expression of lncRNAs in hepatocyte injury by dengue virus-induced antibody dependent enhancement

目的分析登革病毒(DENV)感染L-02细胞中长链非编码RNA(lncRNA)差异表达。方法利用DENV1 NS1抗体和DENV2孵育后感染L-02细胞模拟抗体依赖性感染增强效应(ADE),转录组测序(RNA-Seq)后分析lncRNA表达谱,生物信息学探讨差异表达lncRNA潜在靶基因、miRNA前体预测并利用实时荧光定量PCR(RTqPCR)验证差异表达结果。结果在ADE中得到36个高表达和39个低表达lncRNA,其中n344659潜在靶标基因(ID:10236)具有miRNA前体处理功能;n344462为hsa-let-7a-1和hsa-let-7f-1前体,分别具有肿瘤活性抑制因子作用和抑制Bcl-xL基因表达作用。RT-qPCR显示7个高表达和4个低表达lncRNA(P<0.05)。结论ADE中差异表达lncRNA可能具有重要生物学功能。

Objective To analyze the differential expression of long non-coding RNA(lncRNA)in L-02 cells infected by dengue virus(DENV).Methods Antibody dependent enhancement(ADE)of L-02 cells was established by DENV2 and anti-DENV1 NS1 antibody.lncRNA expression profile was analyzed following transcriptome sequencing(RNA-Seq),and potential target genes and miRNA precursor prediction of differentially expressed lncRNAs were explored using bioinformatics.The differentially expressed lncRNAs were verified by real-time fluorescent quantitative PCR(RT-qPCR).Results There were 36 up-regulated and 39 down-regulated differentially expressed lncRNAs in ADE,of which the potential target gene(gene ID:10236)of n344659 functioned as pre-miRNA processing;n344462 was the precursor of hsalet-7a-1 and hsa-let-7f-1 that respectively suppressed tumor activity and Bcl-xL expression.RT-qPCR showed that 7 upregulated and 4 down-regulated differentially expressed lncRNA(P<0.05).Conclusion The differentially expressed lncRNA might have important biological functions in ADE.