lncRNA-XIST在小鼠急性缺血再灌注肾损伤中的表达情况

Expression of lncRNA-XIST in acute ischemia-reperfusion kidney injury of mice

目的探讨长链非编码RNA-X染色体失活特异转录本(lncRNA-XIST)在小鼠急性缺血再灌注肾损伤(AIKI)中的表达情况。方法选取清洁级雄性C57小鼠90只,将其随机分为对照组、模型组与实验组,各30只。模型组与实验组均根据国际标准建立小鼠AIKI模型,实验组建模成功后注射干扰腺相关病毒lncRNA-XIST siRNA。于lncRNA-XIST siRNA表达2周后的第2、24、48、72小时及第7、14天各处死5只小鼠,采用实时定量PCR法检测小鼠肾组织中lncRNA-XIST的表达水平,于lncRNA-XIST siRNA表达2周后的第48、72 h检测小鼠的血尿素氮(BUN)及血肌酐(Scr)水平。结果在肾皮质及肾髓质中,模型组第2、24、48、72小时及第7、14天的lncRNA-XIST表达水平均高于对照组(P<0.05)。在肾皮质中,从第24小时开始,lncRNA-XIST表达水平呈不断升高趋势。模型组第48、72小时BUN、Scr水平高于对照组及实验组,差异具有统计学意义(P<0.05);实验组第48、72小时血清BUN、Scr水平高于对照组,差异具有统计学意义(P<0.05)。结论lncRNA-XIST在AIKI小鼠肾组织中高表达,特别是在肾皮质中,下调lncRNA-XIST有助于延缓早期急性肾损伤。

Objective To investigate the expression of long non-coding RNA-X inactivation specific transcript(lncRNA-XIST)in acute ischemia-reperfusion kidney injury(AIKI)of mice.Methods A total of 90 clean grade male C57 mice were selected and randomly divided into control group,model group and experimental group,with 30 cases in each group.The model group and the experimental group established the AIKI model according to international standards.After the successful modeling,the experimental group was injected with lncRNA-XIST siRNA.After 2 weeks of lncRNA-XIST siRNA expression,5 mice were killed at 2,24,48,72 h and on 7,14 d respectively.The expression level of lncRNA-XIST in the renal tissue of mice was detected by real-time quantitative PCR.The blood urea nitrogen(BUN)and serum creatinine(Scr)levels were detected at 48 and 72 h after 2 weeks of lncRNA-XIST siRNA expression.Results In the renal cortex and renal medulla,the expression levels of lncRNA-XIST in the model group at 2,24,48,72 h and on 7,14 d were higher than those in the control group(P<0.05).In the renal cortex,the expression level of lncRNA-XIST was increasing from the 24th h.The BUN and SCr levels in the model group were higher than those in the control group and the experimental group at 48 and 72 h,and the differences were statistically significant(P<0.05);the BUN and Scr levels in the experimental group were higher than those in the control group at 48 and 72 h,and the differences were statistically significant(P<0.05).Conclusion lncRNA-XIST is highly expressed in AIKI mice renal tissue,especially in renal cortex.Down regulation lncRNA-XIST can delay early acute renal injury.