蔬菜水果中菊酯类农药残留快速检测的方法研究

Rapid detection method of pyrethroid pesticide residue in vegetables and fruits

为研究一种蔬菜水果中菊酯类农药残留快速检测方法,通过选购市场上常见的胶体金免疫层析产品及菊酯类农药,对蔬菜水果中菊酯农残的快速检测进行前处理步骤的优化,并考察每种胶体金免疫层析产品在果蔬基质样品检测中的灵敏度、特异性、假阳性率、假阴性率和准确度,以实现胶体金免疫层析产品的整体性评价。结果表明,直接稀释法检测大白菜、韭菜和柑橘时,检测限较高,无法满足检测要求;直接提取法适用于大白菜、韭菜和柑橘的快速检测,检测限为2 mg/kg。方法学验证结果表明:以大白菜、韭菜和柑橘作为空白基质的检测限为2 mg/kg,灵敏度均为100%,特异性为100%,假阳性率为0,假阴性率为0。该方法快速准确、灵敏度高,可适用于蔬菜水果中菊酯类农药残留的快速检测。

To study a rapid detection method of pyrethroid pesticides in vegetables and fruits,the pretreatment procedure for rapid detection of tyrosin residues in vegetables and fruits were optimized by choosing colloidal gold immune chromatography products and pyrethroid pesticides from the market.The sensitivity,specificity,false positive rate,false negative rate and accuracy of each colloidal gold immunochromatographic product in the determination of fruit and vegetable matrix samples were investigated to realize the overall evaluation of colloidal gold immunochromatographic products.The results showed that the detection limit was high when using direct dilution method to detect Chinese cabbage,leek and citrus,which was unable to meet the detection requirements.While the direct extraction method was suitable for the rapid detection of Chinese cabbage,leek and citrus,the detection limit was 2 mg/kg.Methodological verification results showed that the detection limit of Chinese cabbage,leek and citrus as blank matrix was 2 mg/kg,the sensitivity was 100%,the specificity was 100%,the false positive rate was 0,and the false negative rate was 0.The method was rapid,accurate and sensitive,could be used for the rapid detection of pyrethroid pesticide residues in vegetables and fruits.