玉米CAMTA基因家族的全基因组分析

Genome-wide Analysis of the CAMTA Gene Family in Maize(Zea mays L.)

钙调素结合转录激活因子(calmodulin binding transcription activator,CAMTA)是一种广泛存在于真核生物中的结构相对保守的钙调素结合型转录因子,对植物生长和胁迫应答起着非常重要的作用。本研究中,我们在玉米基因组发现了7个ZmCAMTA(ZmCAMTA1~ZmCAMTA7)基因。ZmCAMTA2~ZmCAMTA6基因在根和雄穗中显示高表达水平,同时分析了ZmCAMTA基因对多种非生物胁迫(热,冷,盐)的响应。冷胁迫下,ZmCAMTA4基因的转录水平提高了2倍,ZmCAMTA6基因的转录水平提高了3倍。蛋白质理化性质、系统进化及共线性、基因结构等分析表明该基因家族结构、基序、结构域相对保守,共线性分析揭示了ZmCAMTA基因在演化过程中存在全基因组多倍化事件。

The Calmodulin binding transcription activator(CAMTA)is a calmodulin binding transcription factor with a relatively conservative structure,which is widely existed in eukaryotes,and plays the key role for development and abiotic stress response.In this study,total seven Zm CAMTA(ZmCAMTA1~ZmCAMTA7)genes were identified in the maize genome by silico cloning method.The pattern expression of ZmCAMTA2~ZmCAMTA6 showed that increased mRNA levels were exhibited in roots and tassels.Various abiotic stress,such as heat,cold,and salt,were performed,and results displayed that,ZmCAMTA4 and ZmCAMTA6 genes showed accumulated mRNA level under cold treatment.We also analyze the physicochemical properties,system evolution and collinearity,gene structure of the encoded protein.The results showed that the gene family structure,motifs,and domains were relatively conserved.Collinearity analysis suggested the existence of genome-wide physicochemical properties,system evolution and collinearity,gene structure of the encoded protein polyploidy events in the evolution of ZmCAMTA genes.