黄瓜SBP家族基因鉴定与表达分析

Identification and Expression Profiles of SBP Family Genes in Cucumber(Cucumis sativus L.)

SQUAMOSA启动子结合蛋白家族(SBP家族)是植物中特有的一类转录因子,在植物生长发育中发挥重要作用。本研究通过生物信息学方法从黄瓜基因组中鉴定出15个SBP基因,并对这些基因的理化性质、基因结构、系统进化以及不同组织中的表达进行了分析。结果显示黄瓜15个CsSBP基因分布于4条染色体上,分为6个类群,同一个类群中的基因具有相似基因结构和保守基序。表达分析表明。CsSBP9、CsSBP12、CsSBP10、CsSBP3、CsSBP8和CsSBP7在所有组织中都有表达,其余基因在特定组织中表达量比较高,暗示SBP基因在黄瓜生长发育的不同阶段都扮演了重要角色。本研究为进一步鉴定黄瓜SBP基因功能奠定了一定基础。

SQUAMOSA promoter binding protein(SBP),as a plant-specific transcription factor,plays an important role in plant growth development.In this study,15 SBP genes were identified from the cucumber genome by bioinformatics methods,and the physicochemical property,gene structure,phylogeny,and expression of these genes in different tissues were analyzed.The results showed that 15 genes were distributed on 4 chromosomes,and divided into 6 groups.Genes in the same group had similar structure and conservative motifs.Expression analysis shows CsSBP9,CsSBP12,CsSBP10,CsSBP3,CsSBP8 and CsSBP7 are expressed in all tissues,and the other genes are expressed in specific tissues,suggesting that SBP genes play an important role in the growth development of cucumber at different stages.This study lays a foundation for the further identification of cucumber SBP gene function.