都匀茶树种质资源遗传多样性SSR分析及指纹图谱构建

Genetic Diversity Analysis and DNA Fingerprinting Construction with SSR Markers for Tea Germplasm Resources from Duyun

为了解都匀毛尖茶原产地茶树种质资源遗传背景及差异,本试验利用SSR分子标记技术对都匀茶农村11份茶树资源进行了DNA遗传多样性分析。结果表明,15对SSR引物共检测到138个观测等位基因和89.7732个有效等位基因,平均每对引物扩增9.2个观测等位基因和5.9849个有效等位基因,Shannon信息指数及多态性信息含量平均值分别为1.9758和0.8104,11份茶树资源间遗传距离为0.6677~3.7365,说明所有资源的遗传多样性丰富、遗传变异较大。当相似系数为0.30时,11份茶树资源可聚为3个复合聚类。利用5个核心标记获得每份茶树资源的10位数分子指纹编码,构建的分子指纹图谱可快速辨别这些茶树种质资源。

In order to understand the genetic background and genetic differences of different tea germplasm sources,the genetic diversity and molecular fingerprinting of 11 tea germplasms collected from the origin of Duyun Maojian were analyzed by using SSR markers.The results showed that a total of 138 observed alleles and89.7732 effective alleles were found using 15 SSR markers with a mean of 9.2 and 5.9849 per locus.The average values of PIC and Shannon’s information index were 0.8104 and 1.9758,respectively,indicating that there was higher level of diversity in the tested germplasms.The Nei’s genetic distance between 11 tea germplasms varied from 0.6677 to 3.7365,indicating that the genetic variation was large among them.When the threshold of coefficient was 0.30,11 tea germplasms were clustered into three composite categories.10-digit fingerprint database code of each tea germplasm was obtained based on five core SSR markers,DNA fingerprinting constructed with fingerprint database codes can quickly identify these germplasm resources.