野生蓝果忍冬多酚鉴定及其抗氧化、降血糖活性

Polyphenol Composition and Antioxidant and Hypoglycemic Activities in Wild Blue Honeysuckle Fruit

目的:对野生蓝果忍冬进行多酚成分鉴定和抗氧化、抗淀粉酶活性研究。方法:以我国小兴安岭地区的野生蓝果忍冬果实为材料,通过固相萃取技术分离纯化得到花色苷和非花色苷分离液,测定分离液中的化学成分,并测定多酚粗提液中总酚和总花色苷含量,抗氧化活性(1,1-二苯基-2-三硝基苯肼自由基清除能力、铁离子还原能力、2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力、氧自由基吸收能力),抗α-淀粉酶、α-葡萄糖苷酶活性和脂肪酶活性。结果:野生蓝果忍冬果实中含有丰富的花色苷和非花色苷多酚,尤其是矢车菊素-3-葡萄糖苷和山柰酚-3-芸香糖苷含量很高;蓝果忍冬多酚粗提液中总酚含量为82.7 mg/100 g,总花色苷含量为49.8 mg/100 g,具有较强的抗氧化活性;对α-淀粉酶、α-葡萄糖苷酶活性的半抑制质量浓度分别为0.39 mg/mL和0.933 mg/mL,对脂肪酶活性的半抑制质量浓度为12.31 mg/mL,说明蓝果忍冬可以作为一种有前景的淀粉酶抑制剂。

The polyphenol composition and antioxidant and anti-starch hydrolase activities in wild blue honeysuckle fruit collected from the Lesser Khingan Mountains in northeast China were analyzed.Anthocyanin and non-anthocyanin fractions were obtained by solid-phase extraction(SPE)and the chemical composition was determined.Total phenolics and total anthocyanins were also determined in the polyphenol crude extracts,and antioxidant activity was measured by determining 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging capacity,ferric reducing antioxidant power(FRAP),2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radical scavenging capacity,and oxygen radical absorbance capacity(ORAC).In addition,the inhibitory activity ofα-amylase,α-glucosidase and lipase were determined.The results showed that the fruit contained abundant anthocyanins and non-anthocyanin polyphenols,especially cyanidin-3-glucoside and kaempferol-3-rutinoside.The contents of total phenolics and total anthocyanins in the polyphenol crude extracts were up to 82.7 and 49.8 mg/100 g respectively,indicating strong antioxidant activity.The half-maximum inhibitory concentrations(IC50)forα-amylase,α-glucosidase and lipase were 0.39,0.933 and 12.31 mg/mL,respectively.This study demonstrates that blue honeysuckle fruit could be used as a promising inhibitor of starch hydrolase.