30名健康志愿者饮用饮久舒对肝代谢酶的影响

Effects of drinking Yin-Jiu-Shu on liver metabolizing enzymes in 30 healthy volunteers

目的探讨饮久舒植物饮品对肝脏代谢酶的影响。方法选取30名健康志愿者,在饮用饮久舒前及饮用后30、60、120 min时各抽取5 mL静脉血,分离血浆,采用酶联免疫吸附法(ELISA)检测血浆中乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、还原型谷胱甘肽(GSH)、谷胱甘肽巯基转移酶P1(GSTP1)、谷胱甘肽巯基转移酶M3(GSTM3)的水平。结果30名健康志愿者饮用饮久舒后,血浆中ADH、ALDH、GSH、GSTP1、GSTM3五种酶含量均呈升高趋势。与饮用前水平相比,ADH在饮用30 min后组间差异达到有统计学意义的水平(P=0.014),ALDH在饮用120 min后组间差异达到有统计学意义的水平(P=0.016),GSH在饮用60 min后组间差异达到有统计学意义的水平(P=0.031),GSTP1在饮用30 min和60 min后组间差异达到有统计学意义的水平(P=0.046,P=0.012),GSTM3在饮用30 min和60 min后组间差异达到有统计学意义的水平(P=0.008,P=0.039)。结论饮久舒能提高乙醇代谢相关酶和抗氧化物质的水平,可能是其发挥解酒护肝作用的潜在机制。

Objective To explore the effects of a plant beverage,Yin-Jiu-Shu,on liver metabolizing enzymes.Methods A total of 30 healthy volunteers were enrolled.Before,30 min,60 min and 120 min after Yin-Jiu-Shu were administered,5 mL pf venous blood was collected,and plasma was immediately separated to detect the levels of alcohol dehydrogenase(ADH),acetaldehyde dehydrogenase(ALDH),glutathione(GSH),glutathione S-transferase P1(GSTP1),and glutathione S-transferase M3(GSTM3)using enzyme-linked immunosorbent assay(ELISA).Results After Yin-Jiu-Shu was administered,the plasma levels of ADH,ALDH,GSH,GSTP1,GSTM3 significantly increased at 30 minute point(P=0.014),120 minute point(P=0.016),60 minute point(P=0.031),30 and 60 minute points(P=0.046,P=0.012),30 and 60 minute points(P=0.008,P=0.039),respectively.Conclusion Yin-Jiu-Shu can improve the levels of alcohol metabolism related enzymes and antioxidants,which might be its underlying mechanism to promote alcohol metabolism and protect liver.