摘要
目的探讨饮久舒植物饮品对肝脏代谢酶的影响。方法选取30名健康志愿者,在饮用饮久舒前及饮用后30、60、120 min时各抽取5 mL静脉血,分离血浆,采用酶联免疫吸附法(ELISA)检测血浆中乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、还原型谷胱甘肽(GSH)、谷胱甘肽巯基转移酶P1(GSTP1)、谷胱甘肽巯基转移酶M3(GSTM3)的水平。结果30名健康志愿者饮用饮久舒后,血浆中ADH、ALDH、GSH、GSTP1、GSTM3五种酶含量均呈升高趋势。与饮用前水平相比,ADH在饮用30 min后组间差异达到有统计学意义的水平(P=0.014),ALDH在饮用120 min后组间差异达到有统计学意义的水平(P=0.016),GSH在饮用60 min后组间差异达到有统计学意义的水平(P=0.031),GSTP1在饮用30 min和60 min后组间差异达到有统计学意义的水平(P=0.046,P=0.012),GSTM3在饮用30 min和60 min后组间差异达到有统计学意义的水平(P=0.008,P=0.039)。结论饮久舒能提高乙醇代谢相关酶和抗氧化物质的水平,可能是其发挥解酒护肝作用的潜在机制。
Objective To explore the effects of a plant beverage,Yin-Jiu-Shu,on liver metabolizing enzymes.Methods A total of 30 healthy volunteers were enrolled.Before,30 min,60 min and 120 min after Yin-Jiu-Shu were administered,5 mL pf venous blood was collected,and plasma was immediately separated to detect the levels of alcohol dehydrogenase(ADH),acetaldehyde dehydrogenase(ALDH),glutathione(GSH),glutathione S-transferase P1(GSTP1),and glutathione S-transferase M3(GSTM3)using enzyme-linked immunosorbent assay(ELISA).Results After Yin-Jiu-Shu was administered,the plasma levels of ADH,ALDH,GSH,GSTP1,GSTM3 significantly increased at 30 minute point(P=0.014),120 minute point(P=0.016),60 minute point(P=0.031),30 and 60 minute points(P=0.046,P=0.012),30 and 60 minute points(P=0.008,P=0.039),respectively.Conclusion Yin-Jiu-Shu can improve the levels of alcohol metabolism related enzymes and antioxidants,which might be its underlying mechanism to promote alcohol metabolism and protect liver.
作者
向琳
陈腊梅
王婧雯
李海铭
李浩宇
王菊
范玉琛
王凯
XIANG Lin;CHEN Lamei;WANG Jingwen;LI Haiming;LI Haoyu;WANG Ju;FAN Yuchen;WANG Kai(Department of Hepatology,Qilu Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250012,Shandong,China;Institute of Hepatology,Shandong University,Jinan 250012,Shandong,China)
出处
《山东大学学报(医学版)》
CAS
北大核心
2021年第3期81-85,共5页
Journal of Shandong University:Health Sciences
关键词
饮久舒
乙醇脱氢酶
乙醛脱氢酶
还原型谷胱甘肽
氧化应激
Yin-Jiu-Shu
Alcohol dehydrogenase
Acetaldehyde dehydrogenase
Glutathione
Oxidative stress