鸡骨草与毛鸡骨草的比较转录组学分析

Comparative Transcriptomic Study of Abrus cantoniensis and Abrus mollis

目的比较鸡骨草和毛鸡骨草转录组信息,获得两者比较转录组学的差异。方法利用Illumina HiSeqTM 4000高通量测序平台对鸡骨草和毛鸡骨草嫩叶样品进行转录组测序;将基因序列与蛋白库作blastx比对,通过预测编码蛋白框获得编码蛋白和蛋白序列;使用blastp和OrthoMCL软件联用法找出物种间的特有基因和同源基因,开展特有基因KEGG和GO富集分析、单拷贝同源基因分析。结果鸡骨草和毛鸡骨草分别检测出18073、17189个特有基因,同源基因有37881个。特有基因KEGG富集分析发现,鸡骨草的核糖体途径富集基因数量最多且差异最显著,毛鸡骨草中ABC转运蛋白和胞吞作用途径等显著性富集,植物-病原互作在两个物种间都呈现显著性富集;GO富集分析中发现,鸡骨草的胞吞作用调节、囊泡介导的转运调节、核糖体等显著富集,毛鸡骨草中的微管结合复合物、细胞骨架、细胞膜和蛋白磷酸化作用呈现显著性富集。近缘物种比对分析结果显示鸡骨草和毛鸡骨草的物种亲缘关系极为相近。此外,鸡骨草和毛鸡骨草分别发现了2102、1794个简单重复序列(SSR),两者出现频率最高的串联重复单元都为AG/CT。结论研究揭示了鸡骨草与毛鸡骨草在转录组学上的异同,两者具有极为接近的亲缘关系,而特有基因差异揭示了两者在生物代谢、信号传导、发育和功能等方面又存在不同,研究结果将为两者在栽培、生物调控和品质等方面的后续研究奠定基础。

Objective To compare and analyze the transcriptome information of Abrus cantoniensis and A.mollis,and obtained the comparative analysis results of transcriptome between them.Methods Transcriptomes of tender leaves samples of A.cantoniensis and A.mollis were sequenced by using Illumina HiSeqTM 4000 platform.The unigene sequences were compared with the protein library by Blastx,and the coding protein and protein sequences were obtained by predicting the protein coding frames.Blastp combined with OrthoMCL software were used to identify the specific genes and homologous genes in two species.The specific genes were further enriched by KEGG and GO analysis.The single copy homologous genes were analyzed by evolution analysis.Results 18073 and 17189 specific unigenes were obtained respectively in A.cantoniensis and A.mollis with 37881 homologous unigenes.KEGG enrichment analysis of the specific genes showed that the number of ribosomal enrichment genes in A.cantoniensis was the largest and most significant,ABC transporter and endocytosis pathway were significantly enriched in A.mollis,and plant pathogen interaction was significantly enriched in both two species.In GO enrichment analysis,it was found that the endocytosis regulation,vesicle mediated transport regulation and ribosome were significantly enriched in A.cantoniensis.The microtubule associated complex,cytoskeleton,cell membrane and protein phosphorylation in A.mollis were significantly enriched.The comparison results of the two genetically closed species showed that the species similarity of A.cantoniensis and A.mollis was very high.In addition,2102 and 1794 SSRs were found in A.cantoniensis and A.mollis,respectively.The most frequent tandem repeats among them were AG/CT.Conclusion This study revealed the similarities and differences in transcriptomes between A.cantoniensis and A.mollis.They have very close genetic relationship,and the specific gene differences revealed the differences in biological metabolism,signal transduction,development and function between them.These results will lay a foundation for the cultivation,biological regulation and quality research of the two species.