摘要
为探究不同性别尼罗罗非鱼(Oreochromis niloticus)对持续性高温生境的响应,持续监测36℃实验组和28℃对照组的雌鱼和雄鱼的形态学特征变化,并取处理70 d的罗非鱼脑、背部肌肉和鳃组织进行TUNEL染色法分析,选取该实验组和常温对照组个体的背部肌肉进行转录组测序。结果显示:高温处理组的生长发育速度明显迟缓于对照组,雄鱼生长发育比雌鱼快;TUNEL染色法显示在背部肌肉组织中凋亡信号最强,而在其他组织中信号较弱;在高温处理后的雌鱼和雄鱼中分别鉴定出3405个和4645个差异表达基因,上调基因均多于下调基因。对这些差异表达基因的KEGG通路聚类分析发现,雌鱼主要涉及细胞周期、嘌呤代谢和DNA复制等通路,雄鱼在心肌细胞的肾上腺素信号传导、心肌收缩和紧密连接等通路显著富集。这些结果为研究不同性别罗非鱼对高温环境的适应机制提供了分子生物学的基础信息。
In order to investigate the response of male and female Nile tilapia(Oreochromis niloticus)to continuous high temperature habitat,the morphological characteristics of female and male fish were continuously monitored in the 36℃experimental group and the 28℃control group,and the brain,back muscles and gill tissues of female and male fish treated for 70 days were analyzed by TUNEL staining method,and back muscles were selected for transcriptome sequencing.The results showed:The growth rate of the high-temperature treatment group was significantly slower than that of the control group,and the male fish grew faster than the female fish;TUNEL staining showed that the signal of apoptosis was the strongest in back muscle tissues,but weaker in other tissues;The results showed after high temperature treatment,3405 DEGs were identified in females and 4645 DEGs were identified in males,with more up-regulated genes than down-regulated genes.Through the KEGG pathway cluster analysis,female fish were mainly involved in the cell cycle,purine metabolism and DNA replication and other pathways,and male fish were significantly enriched in the pathways of adrenaline signaling,myocardial contraction and tight connection in cardiomyocytes.The research results provide basic information for further exploring the adaptation mechanism of different sex Nile tilapia to high temperature environment.
作者
黄思婕
周艳
魏亚丽
鲁纪刚
陈良标
HUANG Sijie;ZHOU Yan;WEI Yali;LU Jigang;CHEN Liangbiao(International Research Center for Marine Bioscience,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2021年第3期426-434,共9页
Journal of Shanghai Ocean University
基金
国家重点研发计划(2018YFD0900600)
上海市科学技术委员会“一带一路”国际联合实验室项目(19590750500)
上海市教育委员会科研创新计划(2017-01-07-00-10-E00060)。
关键词
尼罗罗非鱼
高温处理
肌肉
转录组
Nile tilapia
high temperature treatment
muscle
transcriptome