裸藻多糖的分离纯化、单糖组成及其抗氧化活性

Isolation,purification,monosaccharide composition and antioxidant activity analysis of Euglena gracilis polysaccharides

以裸藻为原料,将粗提后的裸藻多糖(EGPS)经DEAE-纤维素和Sephadex G-100柱层析进行分离纯化,得到3个主要组分EGPS1-A、EGPS1-B和EGPS3-A,并对其进行纯度鉴定和抗氧化活性分析,将纯化后抗氧化活性较好的裸藻多糖进行分子量测定、单糖组成分析。纯度鉴定结果表明柱层析后得到的3个多糖组分纯度较高,红外光谱表明其均具有典型的糖类特征吸收峰。抗氧化实验表明3个多糖组分中EGPS1-A的综合抗氧化活性最高,分子量为136.8 ku,单糖组成包括甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖、岩藻糖,单糖组分间物质的量比是0.072∶0.430∶0.049∶0.010∶0.260∶0.620∶0.530∶0.180∶1.010。

Euglena gracilis was used as raw material.EGPS was separated and purified using DEAE cellulose colum and Sephadex G-100.EGPS1-A,EGPS1-B,EGPS3-A were obtained.On this basis,the purity identification and antioxidant activity of polysaccharide fractions were analyzed.The polysaccharides with better antioxidant activity were determined by molecular weight and monosaccharide composition.The purity identification results showed the purity of the three polysaccharides was high.Infrared detection confirmed that the purity of the three polysaccharides had characteristic absorption peaks of polysaccharides.The results of antioxidant activity showed EGPS1-A had the highest antioxidant activity among the three polysaccharides.The molecular weight of EGPS1-A was 136.8 ku and it consisted of 9 kinds of sugars such as mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose,xylose,arabinose,fucose,and the composition ratios were 0.072∶0.430∶0.049∶0.010∶0.260∶0.620∶0.530∶0.180∶1.010.