禽蛋中氟苯尼考代谢物快速检测方法的建立

Rapid Detection of Florfenicol Metabolites in Poultry Eggs

基于免疫层析技术建立禽蛋中氟苯尼考残留标志物(氟苯尼考与氟苯尼考胺之和)的快速检测方法。采用乙酸乙酯对禽蛋中氟苯尼考及其主要代谢物氟苯尼考胺进行提取,并用二氯乙酸酐将提取液中的氟苯尼考胺衍生化为氟苯尼考,最后用氟苯尼考胶体金检测卡进行检测。结果表明,氟苯尼考胺在乙酸乙酯体系中成功衍生化为氟苯尼考,氟苯尼考及氟苯尼考胺检出限分别为1、3μg/kg。另外,通过对实际阳性鸡蛋样品及加标样品进行检测,其结果与国标方法测量结果基本一致。本方法具有简便、快速、低成本等特点,适合大批量样品的现场筛查,提高监管效率。

A rapid method for the detection of a marker residue for florfenicol and its major metabolite florfenicol-amine in poultry eggs was established using immunochromatography.Florfenicol and florfenicol-amine were extracted from poultry eggs with ethyl acetate,and florfenicol-amine was derived into florfenicol by dichloroacetic anhydride.Finally,florfenicol was detected by a colloidal gold test strip.The results showed that florfenicol-amine was successfully converted into florfenicol in ethyl acetate,and the detection limits of florfenicol and florfenicol-amine were 1 and 3μg/kg,respectively.In addition,the method was successfully applied to actual positive egg samples and spiked samples with results consistent with those obtained by the existing national standard method.This method has the characteristics of simple operation,fast detection and low cost,which is suitable for the on-site screening of massive samples,improving food safety supervision efficiency.