厚朴酚对水杨酸钠致妊娠小鼠妊娠损伤的影响

Effects of Magnolol on Sodium Salicylate-Induced Pregnancy Damage of Pregnant Mice

本研究旨在探究厚朴酚对高浓度水杨酸钠引起的妊娠小鼠妊娠损伤的修复作用及其作用机制。选取96只妊娠小鼠,随机均分为4组,分别为对照组、模型组(Ⅰ组)和修复组(Ⅱ和Ⅲ组),每组6个重复,每个重复4只。对照组给予正常饮水[含1%二甲基亚砜(DMSO)]并灌胃生理盐水,Ⅰ、Ⅱ、Ⅲ组分别于正常饮水(含1%DMSO)中补充0、80和800μmol/L厚朴酚,并每天灌胃280 mg/kg水杨酸钠。试验持续9 d,其中妊娠第1天至第4天进行灌胃试验。结果表明:与对照组相比,Ⅰ组子宫全重和胚胎数显著降低(P<0.05);妊娠第4天时,子宫内膜组织还原型辅酶/醌氧化还原酶1(NQO1)基因相对表达量显著下调(P<0.05),而白血病抑制因子受体(LIFR)基因相对表达量显著上调(P<0.05);妊娠第9天时,血清中超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、子宫内膜组织E-钙黏蛋白(CDH1)和核因子E2相关因子2(NRF2)基因相对表达量均显著降低(P<0.05),而血红素加氧酶-1(HO-1)和同源盒基因-A10(HOXA-10)基因相对表达量显著上调(P<0.05)。与Ⅰ组相比,Ⅲ组子宫全重和胚胎数显著上调(P<0.05);妊娠第9天时,血清中SOD活性和T-AOC显著上调(P<0.05)。此外,在子宫内膜组织中,妊娠第4天时,与Ⅰ组相比,Ⅲ组LIFR基因相对表达量显著下调(P<0.05);妊娠第9天时,CDH1和NRF2基因相对表达量显著上调(P<0.05),HO-1和HOXA-10基因相对表达量显著下调(P<0.05)。综上所述,厚朴酚可缓解高浓度水杨酸钠所引起的妊娠小鼠妊娠和胚胎损失,这一作用可能与其在妊娠胚胎定植期和妊娠中期对子宫内膜的适应性调节相关。

This study was conducted to explore the effects of magnolol on sodium salicylate-induced pregnancy damage of pregnant mice and its mechanism.A total of 96 pregnant mice were randomly divided into 4 groups,namely the control group,the model group(groupⅠ)and the repair groups(groupsⅡandⅢ),with 6 replicates in each group and 4 mice per replicate.The control group was given by distilled water(1%dimethylsulfoxide)and gavage normal saline.GroupsⅠ,Ⅱ,andⅢwere given by distilled water(1%dimethylsulfoxide)with 0,80 and 800μmol/L magnolol and gavage 280 mg/kg sodium salicylate per day.The test lasted for 9 days and gavage test was carried out from the first day of pregnancy(GD1)to GD4.The results showed that compared with the control group,in groupⅠ,the gravid uterus weight and fetus number were significantly reduced(P<0.05),and the relative expression level of NAD(P)H dehydrogenase 1(NQO1)was significantly decreased(P<0.05),while the relative expression level of leukemia inhibitory factor receptor(LIFR)was significantly increased(P<0.05)in the endometrium on GD4.The serum superoxide dismutase(SOD)activity,total antioxidant capacity(T-AOC),endometrial relative expression levels of E-cadherin(CDH1)and nuclear factor E2 related factor 2(NRF2)were significantly reduced(P<0.05),while the relative expression levels of heme oxygenase-1(HO-1)and homeobox gene A10(HOXA10)were significantly up-regulated(P<0.05)on GD9 in groupⅠ.Compared with groupⅠ,the gravid uterus weight and fetus number in groupⅢwere significantly up-regulated(P<0.05),and the serum SOD activity and T-AOC in pregnant mice on GD9 were significantly up-regulated(P<0.05).In addition,compared with groupⅠ,the relative expression level of LIFR on GD4 in endometrium in groupⅢwas significantly down-regulated(P<0.05),and the relative expression levels of CDH1 and NRF2 were significantly up-regulated on GD9(P<0.05),the relative expression levels of HO-1 and HOXA10 were significantly decreased on GD9(P<0.05).In summary,these results indicate that magnolol can alleviate pregnancy damage and embryo loss caused by high concentration of sodium salicylate.These effects may be related to the adaptive regulation of magnolol on the endometrium during embryo implantation and mid-gestation.