饲粮添加驴源罗伊氏乳杆菌对断奶驴驹生长性能、营养物质表观消化率、血清免疫球蛋白含量及直肠菌群结构的影响

Effects of Dietary Donkey-Derived Lactobacillus reuteri on Growth Performance,Nutrient Apparent Digestibilities,Serum Immunoglobulin Contents and Rectum Microbiota Structure of Weaning Donkey Foals

本试验旨在研究饲粮添加驴源罗伊氏乳杆菌对断奶驴驹生长性能、营养物质表观消化率、血清免疫球蛋白含量及直肠菌群结构的影响。选取5~6月龄体况良好的断奶驴驹10头,平均体重为(113.05±14.66)kg,随机分为2组,每组5头。对照组(CT组)饲喂基础饲粮;试验组(LA组)在基础饲粮中添加罗伊氏乳杆菌,按1×1011 CFU/kg添加于精饲料中。预试期7 d,正试期60 d。结果表明:1)对照组和试验组的体重、平均日增重、料重比均无显著差异(P>0.05)。试验组的粗饲料采食量显著低于对照组(P<0.05)。2)对照组和试验组的干物质(DM)、粗蛋白质(CP)、粗脂肪(EE)、酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)表观消化率均无显著差异(P>0.05)。与对照组相比,试验组的ADF表观消化率提高了6.2%,NDF表观消化率提高了3.4%。3)对照组和试验组的血清免疫球蛋白G(IgG)和免疫球蛋白M(IgM)含量均无显著差异(P>0.05)。试验组的血清免疫球蛋白A(IgA)含量显著高于对照组(P<0.05)。4)饲粮添加罗伊氏乳杆菌对断奶驴驹直肠菌群α-多样性无显著影响(P>0.05),饲粮添加罗伊氏乳杆菌提高了断奶驴驹直肠菌群门水平上拟杆菌门和属水平上未鉴定瘤胃球菌科的相对丰度(P<0.05)。由此可见,饲粮添加罗伊氏乳杆菌能减少断奶驴驹粗饲料的摄入,提高ADF和NDF表观消化率,增加血清IgA含量,提高直肠拟杆菌门和未鉴定瘤胃球菌科的相对丰度。

The aim of this study was to investigate the effects of dietary donkey-derived Lactobacillus reuteri on growth performance,nutrient apparent digestibilities,serum immunoglobulin contents and rectum microbiota structure of weaning donkey foals.Ten healthy weaning donkey foals aged from 5 to 6 months with average body weight of(113.05±14.66)kg were selected and randomly assigned into 2 groups with 5 donkeys per group.The control group(CT group)was fed a basal diet;the experimental group(LA group)was fed the basal diet supplemented with Lactobacillus reuteri,which supplemented 1×1011 CFU/kg in concentrate.The pre-experimental period lasted for 7 days,and the experimental period lasted for 60 days.The results showed as follows:1)there were no significant differences in body weight,average daily gain and feed to gain ratio between control group and experimental group(P>0.05).The roughage feed intake of the experimental group was significantly lower than that of the control group(P<0.05).2)There were no significant differences in apparent digestibilities of dry matter(DM),crude protein(CP),ether extract(EE),acid detergent fiber(ADF),neutral detergent fiber(NDF)between control group and experimental group(P>0.05).Compared with the control group,the ADF apparent digestibility of the experimental group increased by 6.2%,and the NDF apparent digestibility of the experimental group increased by 3.4%.3)There were no significant differences in contents of immunoglobulin G(IgG)and immunoglobulin M(IgM)in serum between control group and experimental group(P>0.05).The serum immunoglobulin A(IgA)content of the experimental group was significantly higher than that of the control group(P<0.05).4)Dietary Lactobacillus reuteri had no significant effect on the rectal microbialα-diversity of weaning donkey foals(P>0.05),and dietary Lactobacillus reuteri significantly increased the relative abundances of Bacteroidetes at phylum level and unidentified Ruminococcaceae at genus level in rectum(P<0.05).In conclusion,dietary Lactobacillus reuteri can reduce the roughage intake of weaning donkey foals,increase the apparent digestibilities of ADF and NDF,improve the serum IgA content,and increase the relative abundances of Bacteroidetes and unidentified Ruminococcaceae in rectum.