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替米沙坦对CVB3诱导的病毒性心肌炎小鼠的保护作用 被引量:1

Protective Effect of Telmisartan on Mice with Viral Myocarditis Induced by CVB3
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摘要 该文探讨了替米沙坦对柯萨奇B3(Coxsackie B3,CVB3)病毒诱导的病毒性心肌炎小鼠的保护作用。该研究将60只小鼠随机分为对照组、模型组、观察组,每组20只。将CVB3病毒溶解后腹腔注射制作模型,观察组小鼠给予替米沙坦喂食,7天后处死。观察比较3组小鼠心肌组织病理情况,使用试剂盒检查各组超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)及谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-Px)的水平;使用酶联免疫检测白细胞介素-1β(interleukin-1β,IL-1β)、γ干扰素(interferon-γ,IFN-γ)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)。结果显示,观察组小鼠心肌组织细胞排列趋近于规律,且细胞间缝隙较小,同时炎症细胞较少。模型组小鼠心肌组织中氧化应激指标MDA较对照组显著升高,GSH-Px以及SOD较对照组显著降低(P<0.01);观察组小鼠心肌组织中MDA较模型组显著降低,GSH-Px以及SOD较模型组显著升高(P<0.01)。模型组小鼠心肌组织中IFN-γ、TNF-α以及IL-1β含量较对照组显著升高(P<0.01);观察组小鼠心肌组织中炎症因子含量较模型组显著降低(P<0.01)。模型组小鼠心肌细胞iNOS、p-p65、TLR4蛋白表达水平较对照组均显著升高(P<0.01);观察组小鼠心肌细胞iNOS、p-p65、TLR4蛋白表达水平较模型组均显著降低(P<0.01)。模型组小鼠心肌细胞中Nrf2相关蛋白表达水平较对照组显著降低(P<0.01);观察组小鼠心肌细胞中Nrf2相关蛋白表达水平较模型组显著升高(P<0.01)。该研究得出结论:针对病毒性心肌炎的小鼠模型,早期使用替米沙坦后可以通过参与氧化应激以及炎症反应过程来达到减轻心肌受损的目的。 The aim of this study was to investigate the protective effect of telmisartan on CVB3(Coxsackie B3)-induced viral myocarditis in mice.Sixty mice were randomly divided into control group,model group and observation group,with 20 mice in each group.The CVB3 virus was dissolved and injected into the abdominal cavity to make a model,and the observation group mice were fed with telmisartan for seven days and then sacrificed.This article observed the pathological conditions of the myocardial tissues of the three groups of mice,and detected the levels of SOD,MDA,GSH-Px,IL-1β,IFN-γand TNF-αby enzyme-linked immunoassay in each group.The arrangement of myocardial cells in the observation group tended to be regular,with smaller gaps between cells and fewer inflammatory cells;the level of oxidative stress indicator MDA in the myocardial tissue of the model group was significantly higher than that of the normal group,while the levels of GSH-Px and SOD were significantly lower than those of the normal group(P<0.01);MDA level in the myocardial tissue of the observation group was significantly lower than that of the model group,while GSH-Px and SOD were significantly higher than that of the model group(P<0.01).The levels of IFN-γ,TNF-αand IL-1βin the myocardial tissue of the model group were significantly higher than those of the normal group(P<0.01),while the levels of inflammatory factors in the myocardial tissue of the observation group were significantly lower than those of the model group(P<0.01).The levels of iNOS,p-p65 and TLR4 in myocardial cells of the model group were significantly higher than those in the control group(P<0.01),while the levels of iNOS,p-p65,and TLR4 in the myocardial cells of the observation group were higher than those of the model group(P<0.01).The level of Nrf2-related protein in the cardiomyocytes of the model group was significantly lower than that of the control group(P<0.01),while the level of Nrf2-related protein in the cardiomyocytes of the observation group was lower than that of the model group(P<0.01).For the mice model of viral myocarditis,early use of telmisartan can reduce myocardial damage by participating in oxidative stress and inflammatory reaction process.
作者 袁玲 甘继宏 职宁 高玉梅 刘丹玉 YUAN Ling;GAN Jihong;Zhi Ning;GAO Yumei;LIU Danyu(Outpatient Department of the Eighth Affiliated Hospital(North District)of Xinjiang Medical University,Urumqi 830014,China;Cardiology Department of General Hospital of Xinjiang Military Region,Urumqi 830000,China;Internal Medicine Department of the Eighth Affiliated Hospital(North District)of Xinjiang Medical University,Urumqi 830014,China;Medical Department of the Eighth Affiliated Hospital(North District)of Xinjiang Medical University,Urumqi 830014,China;Outpatient Department of the Eighth Affiliated Hospital of Xinjiang Medical University(Southern District),Urumqi 830049,China)
出处 《中国细胞生物学学报》 CAS CSCD 2021年第5期956-962,共7页 Chinese Journal of Cell Biology
基金 全军医药卫生科研基金(批准号:CLZ15JA09)资助的课题。
关键词 替米沙坦 病毒性心肌炎 柯萨奇B3病毒 氧化应激 telmisartan viral myocarditis Coxsackie B3 virus oxidative stress
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