MBD3调节干细胞多能性和重编程机制的研究进展

Progress on the Mechanism of MBD3 Regulating the Pluripotency of Stem Cells and Reprogramming

MBD3(methyl CpG binding domain 3)是甲基CpG结合域蛋白家族的成员之一,也是NuRD(nucleosome remodeling and deacetylase complex)的核心亚单位之一。MBD3蛋白可以结合非甲基化DNA,通过MBD蛋白结构域或与NuRD结合发挥作用。MBD3通过参与调节染色质结构和激活转录过程,调节胚胎干细胞的多能性和谱系分化,对于胚胎发育和分化十分关键。MBD3在体细胞和神经干细胞重编程中也发挥着重要作用。此外,在缺氧环境下MBD3还能影响细胞代谢调控。该文围绕MBD3诱导DNA去甲基化、调节染色质结构、调控转录、调节胚胎干细胞的多能性和谱系分化、在重编程中的作用以及缺氧环境中的对细胞代谢的影响等展开论述,以期为多能干细胞的表观遗传研究及重编程技术的优化提供参考。

MBD3(methyl CpG binding domain 3)is a member of the MBD(methyl CpG binding domain)protein family and a core subunit of the NuRD(nucleosome remodeling and deacetylase complex).The MBD3 protein can bind to the unmethylated DNA,functioning through the MBD domain or in combination with NuRD.MBD3 is a key protein for embryonic development and differentiation due to involving in scaffolding chromatin structure and activating transcription processes to regulate the pluripotency and lineage differentiation of embryonic stem cells.MBD3 also plays an important role in the somatic cell and neural stem cell reprogramming.Furthermore,MBD3 affects cell metabolism under hypoxic environment.This paper highlights the roles of MBD3 in DNA demethylation,chromatin structure modelling,transcription regulation,pluripotency maintaining and lineage differentiation of embryonic stem cells,the somatic cell reprogramming and the effect on cell metabolism under hypoxia,aiming to provide a reference for the epigenetic research of embryonic stem cells and the optimization of reprogramming technology.