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寄主诱导的基因沉默在增强植物真菌病害抗性方面的研究进展 被引量:4

Research progress on host-induced gene silencing to promote plant resistance against fungal disease
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摘要 寄主诱导的基因沉默(host-induced gene silencing,HIGS)技术以RNAi技术为基础,可从反向遗传学角度研究基因功能,同时可创制具有持久抗性且稳定遗传的抗病品种,为增强植物抗病性提供了新的视角。本文阐明了HIGS技术的原理,总结了近年来该技术在增强植物真菌病害抗性方面最新的研究进展,分析了HIGS技术的优、缺点,并对HIGS技术的应用前景进行了展望。 Host-induced gene silencing(HIGS)is a new technology based on RNA interference(RNAi).HIGS can identify gene functions from the view of reverse genetics and create disease resistant varieties with persistent resistance and stable inheritance.Thus,HIGS provides a new perspective for promoting plant disease resistance.In the current paper,we discuss the principles of HIGS technology,summarize the latest research progress in enhancing plant resistance against fungal diseases,and analyze the advantages and disadvantages of HIGS.Future prospects of HIGS technology are also discussed,with a focus on in-depth study of plant resistance.
作者 冯丹丹 邓蕾 汪祖鹏 潘慧 李文艺 钟彩虹 李黎 Feng Dan-Dan;Deng Lei;Wang Zu-Peng;Pan Hui;Li Wen-Yi;Zhong Cai-Hong;Li Li(Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Engineering Laboratory for Kiwifruit Industrial Technology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China;University of Chinese Academy of Sciences, Beijing 100049, China)
出处 《植物科学学报》 CAS CSCD 北大核心 2021年第3期316-323,共8页 Plant Science Journal
基金 国家自然科学基金项目(31701974,31901980) 武汉市科技局前资助科技计划(2018020401011307)。
关键词 寄主诱导的基因沉默 真菌病害 抗病性 Host-induced gene silencing Fungal diseases Disease resistance
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  • 1陈捷胤,戴小枫.棉花对黄萎病的抗病机制研究进展[J].分子植物育种,2005,3(3):427-435. 被引量:33
  • 2安泽伟,黄华.一种提取橡胶树叶中总DNA的方法[J].植物生理学通讯,2005,41(4):513-515. 被引量:66
  • 3郑武,陈继圣,郑士琴,刘丽华,刘淑集,鲁国东,周洁,王宗华.稻瘟菌Rho族蛋白及其编码基因表达特点[J].植物病理学报,2006,36(4):328-336. 被引量:6
  • 4焦春香.活性氧与植物防卫反应[J].大理学院学报(综合版),2006,5(10):73-76. 被引量:8
  • 5Halpin C, Cooke S E, Barakate A, Amrarfi A E and Ryan M D, 1999. Self-processing 2A-polyproteins-a system for co- ordinate expression of multiple proteins in transgenic plants. Plant Journal, 7(4): 453-459.
  • 6Halweg C, Thompson WF and Spiker S, 2005. The rb7 matrix attachment region increases the likelihood and magnitude of transgene expression in tobacco cells: A flow cytometric study. Plant Cell, 17:418-429.
  • 7Johnston K. Clements A, Venkatammani R N, Trievel R C and Marmorstein R, 2000. Coexpression of proteins in bacteria using T7-based expression plasmids: Expression of heteromeric cell- cycle and transcriptional regulatory complexes. Protein Expression and Purification, 20:435-443.
  • 8Koncz C and Schell J, 1986. The promoter of the TL-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of Agrobacteritma binary vector. Molecular and General Genetics, 204:383-386.
  • 9Lee H Y and Khosla C, 2007. Bioassay-guided evolution of glycosylated macrolide antibiotic in Escherichia coli. Plos Biologyl, 5(2): 243-250.
  • 10Luke G A, 2012. Translating 2A research into practice, Agbo E C (ed.), Innovations in Biotechnology. InTech, Rijeka, Croatia, pp. 161-186.

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