大豆油体蛋白基因家族的生物信息学分析

Bioinformatics Analysis of Glycine Max Oleosin in Soybean

油体蛋白(oleosin)是植物中的一类贮藏蛋白,主要作用是维持油体的稳定和贮藏脂质。通过生物信息学方法,对大豆油体蛋白(Glycine max oleosin, Gmole)基因家族中12个成员(编号Gmole1~Gmole12)的理化性质、结构特点等进行预测分析并建立进化树。结果表明:12个成员分布在大豆的8条染色体上,没有基因簇的形成;等电点均在5.08~5.18;根据相对分子质量大小,可以分为H型和L型2类;Gmole基因家族成员均具有高度保守的疏水区以及脯氨酸节结构,推测该结构是油体蛋白行使锚定油体功能的主要结构。Gmole基因家族成员氨基酸序列的保守区存在2~3个跨膜结构,推测可形成U型探针的结构锚定油体。Gmole基因家族中Gmole8的三级结构无螺旋,其余成员均为1条单螺旋或双螺旋。聚类分析结果推测Gmole1、Gmole2、Gmole3和Gmole6具有改变大豆种子中的油脂组分含量的功能。

Oil body protein(oleosin) is a kind of storage protein in plants, whose main function is to maintain the stability of oil and to store lipid. Through bioinformatics methods, this study predicted and analyzed the physicochemical properties and structural characteristics of 12 members of the Glycine Max oleosin(Gmole) gene family, and established an evolutionary tree. The results show that the 12 members are distributed on 8 chromosomes of soybean, and there are no genetic clusters. The isoelectric points are between 5.08-5.18. According to the relative molecular mass, the 12 members can be divided into two types: H type and L type. The members of the Gmole gene family all have highly conserved hydrophobic regions and proline node structures, which are supposed to be the main structures of oil body protein to anchor oil body. There are 2-3 transmembrane structures in the conserved region of amino acid sequence of Gmole gene family members, which suggests that the structures of U-probe anchored oil body could be formed. In gmole gene family, the tertiary structure of Gmole8 has no helix, and other members are single helix or double helix. The results of cluster analysis suggest that Gmole1, Gmole2, Gmole3 and Gmole6 could change the content of oil components in soybean seeds.