人参锈腐菌和根腐菌对人参皂苷类物质的化学趋向性响应

Chemotaxis Response of Different Ginsenosides and Aglycones to Cylindrocarpon destructans and Fusarium solani

为了考察人参皂苷类物质是否能作为趋化物质在人参锈腐菌(Cylindrocarpon destructans)和人参根腐菌(Fusarium solani)侵染过程中发挥作用。采用平板法、孢子悬滴萌发法和菌丝干重法研究人参二醇型苷元、原人参二醇型苷元、Re和Rd的不同梯度浓度(500,50,5,0.5 mg/L)、pH(5,6,7,8)和不同趋化温度(20,25,30℃)以及不同趋化时间(2,3,4,5,6,7 d)下对人参锈腐菌和人参根腐菌影响。结果表明:人参二醇型苷元、原人参二醇型苷元、Re和Rd对人参锈腐菌和人参根腐菌均具有不同化学趋向性作用,且其最优趋化条件各不相同,在最优趋化条件下,原人参二醇型苷元作用于人参根腐菌时,趋化性指数(CMI)最大,达到1.200 0;原人参二醇型苷元作用于人参锈腐菌时,孢子萌发率和菌丝干重均达到最大,为81.67%和0.396 7 mg/mL。因此,人参锈腐菌与人参根腐菌对人参二醇型苷元、原人参二醇型苷元、Re和Rd具有不同化学趋向性响应。

The study aims to investigate whether ginsenosides can act as chemotactic substances in the process of Cylindrocarpon destructans and Fusarium solani infection. Plate method, spore suspension method and mycelial dry weight method were used to study the effects of panaxadiol aglycon, protopanaxadiol aglycon, Re and Rd of different concentration(500, 50, 5, 0.5 mg/L),pH(5, 6, 7, 8), temperature(20, 25, 30 ℃) and time(2, 3, 4, 5, 6, 7 d) on Cylindrocarpon destructans and Fusarium solani. Panaxadiol aglycon, protopanaxadiol aglycon, Re and Rd had different chemotaxis effects on Cylindrocarpon destructans and Fusarium solani, and the optimal chemotaxis conditions were different. Under the optimal chemotaxis conditions, when the protopanaxadiol aglycon acted on Fusarium solani, the CMI was the highest, which was 1.200 0, and when the protopanaxadiol aglycon acted on Cylindrocarpon destructans, spore germination rate and dry weight of mycelium were the highest, which were 81.67% and 0.396 7 mg/mL, respectively. Cylindrocarpon destructans and Fusarium solani had different chemotactic responses to panaxadiol aglycon, protopanaxadiol aglycon, Re and Rd.