猪毛蒿精油及其活性成分萜品-4-醇对黄曲霉的抑制作用研究

Study on the Inhibitory Effect of Essential Oil from Artemisia scoparia and Its Active Ingredient Terpine-4-ol on Aspergillus flavus

为了探究猪毛蒿Artemisiascoparia精油及其活性成分萜品-4-醇对黄曲霉Aspergillusflavus的抗菌活性及其在花生贮藏保鲜中的应用前景,通过气相色谱-质谱联用(Gas Chromatography-Mass Spectrometer,GC-MS)技术对猪毛蒿精油进行成分分析;采取熏蒸法检测猪毛蒿精油及萜品-4-醇对黄曲霉菌丝生长,孢子萌发和花生体内霉菌数的影响;通过扫描电镜观察猪毛蒿精油和萜品-4-醇对黄曲霉显微结构的影响。结果显示猪毛蒿精油由24种化合物组成,其主要成分为桉叶油醇(43.8%),萜品-4-醇在猪毛蒿精油中的含量为2.81%;猪毛蒿精油和萜品-4-醇可显著抑制黄曲霉菌丝的生长,并导致菌丝皱缩、扭曲和凹陷;体内抑菌试验发现,猪毛蒿精油和萜品-4-醇能显著降低花生体内霉菌数。

In order to explore the antibacterial activity of essential oil from Artemisia scoparia and its active ingredient terpinen-4-ol against Aspergillus flavus and its application prospects in the storage and preservation of peanuts,the composition of essential oil from A.scoparia were detected by gas-phase mass spectrometry(GC-MS);Fumigation method was used to detect the effects of essential oil from A.scoparia and terpinen-4-ol on A.flavus mycelial growth and mold counts in peanuts;Scanning electron microscope(SEM)was used to observe the effects of essential oil from A.scoparia and terpinen-4-ol on A.flavus influence of microstructure.The results showed that essential oil from A.scoparia contains 24 compounds,the main component of which is eucalyptol(43.8%),the content of terpinen-4-ol in A.scoparia essential oil was 2.81%;Essential oil from A.scoparia and terpinen-4-ol can significantly inhibit the growth of A.flavus hyphae,and cause the hyphae to shrink,twist and dent;In vivo antibacterial experiments found that essential oil from A.scoparia and terpinen-4-ol can significantly reduce the number of mold in peanuts.