双尾新小绥螨种群取食猎物偏好性

Study on predation preference of Neoseiulus bicaudus strain in laboratory

【目的】研究添加人工饲料后不同食料饲养的双尾新小绥螨实验室种群对不同猎物的取食影响,分析人工饲料在双尾新小绥螨实验室种群扩繁和释放中的作用。【方法】采用小室法观察在有无人工饲料条件下,自然猎物、替代猎物和人工饲料饲养的双尾新小绥螨种群对目标猎物的捕食选择性和取食量差异,采用偏好性系数理论公式和影响系数,分析人工饲料与猎物共存时对双尾新小绥螨捕食作用的影响。【结果】人工饲料的添加导致双尾新小绥螨的自然猎物饲养种群和替代猎物饲养种群对替代猎物腐食酪螨的取食率降低;3个种群对自然猎物土耳其斯坦叶螨的选择及取食率无显著影响。自然猎物饲养种群对土耳其斯坦叶螨的取食量显著高于其他两种饲养种群,无人工饲料时,自然猎物饲养种群对土耳其斯坦叶螨取食率最高可达83.33%,提供人工饲料时,自然猎物饲养种群对土耳其叶螨的取食率最高可达79.33%;替代猎物饲养种群对腐食酪螨的取食量显著高于自然猎物和人工饲料饲养种群,无人工饲料存在时,替代猎物饲养种群对腐食酪螨的取食率最高可达63.33%。【结论】人工饲料作为双尾新小绥螨的营养补充添加,并不会影响其对土耳其斯坦的防治效果,实验室饲养中作为营养补充添加,可以降低对替代猎物腐食酪螨的消耗。双尾新小绥螨前期的取食经历对其后期的猎物选择及取食量具有重要的作用。

【Objective】Artificial diet could play a role in artificial rearing and population keeping of natural enemy insects by adding nutrients.In this study,the artificial diet adding influence of predation of N.bicaudus were tested among several predatory mite lab strains from different experience of feeding.The objective was to evaluate the artificial diet for predatory mite of lab-strains in artificial rearing and releasing in the field.【Method】Three strains of N.bicaudus(1 fed by natural prey;2 fed by alternative prey;3 fed by artificial diet)were tested on two different conditions(with/without artificial diet).We observed the predation and selectivity of predatory mite in plexiglass chambers.Prey preference and influence coefficient were used to analyze the effect of artificial diet on predation of N.bicaudus.【Result】After adding artificial diet,thefeeding rates of N.bicaudus to alternative prey(T.putrescentiae)were decreased.Differently,the adding of artificial would not interfere the feeding rates of N.bicaudus to natural prey(T.turkestani).The predation of N.bicaudus to T.turkestani was the highest when they fed on natural prey among all strains,when there is no artificial feed,the feeding rate can reach 83.33%,when artificial feed is provided,the feeding rate can reach 79.33%.Similarly,the predation of N.bicaudus to T.putrescentiae was the highest when they fed on alternavitive prey among all strains,when there is no artificial feed,the feeding rate can reach 63.33%.【Conclusion】Artificial diet could provide nutrition for N.bicaudus in field to keep the population of predatory mite in lab which would not interfere the control effect on T.turkestani.In addition,artificial diet could help to relieve stress of prey in the artificial rearing of predatory mite by providing extra nutrition.The feeding experience of N.bicaudus was very important for selection and predation of predatory mite.