糖尿病视网膜病变患者PERK、CHOP和IRE表达水平变化意义分析

Significance of Changes of PERK,CHOP and IRE Expression in Patients with Diabetic Retinopathy

目的:探讨糖尿病视网膜病变患者血清PERK、CHOP和IRE水平及意义。方法:选取2016年1月—2018年8月在许昌市第五人民医院治疗的糖尿病患者210例,其中有糖尿病视网膜病变(DR)患者60例(DR),其中非增殖期视网膜病变(NPDR)患者34例,增期期视网膜病变(PDR)患者26例;无视网膜病变患者150例(DM组),同时选取健康者100例作为对照组。采用Western blot检测视网膜中PERK、CHOP和IRE表达。结果:DR组血清PERK和IRE相对表达量分别为(1.382±0.112)和(0.880±0.102),明显高于对照组和DM组(P<0.05);DR组血清CHOP相对表达量为(1.011±0.110),明显高于对照组(P<0.05),而与DM组比较无统计学意义(P>0.05);DR组PDR患者血清PERK和IRE相对表达量分别为(1.404±0.110)和(0.922±0.104),明显高于NPDR患者(P<0.05);DR组NPDR和PDR患者CHOP相对表达量比较差异无统计学意义(P>0.05);DR组患者PERK和IRE呈正相关(r=0.402,P<0.05);CHOP与PERK、IRE无明显相关性(P>0.05)。结论:显示糖尿病视网膜病变患者PERK、CHOP和IRE表达明显升高,可能在病变过程中有重要作用。

Objective:To investigate the serum levels of PERK,CHOP and IRE in patients with diabetic retinopathy and their significance.Methods:210 patients with diabetes were treated in the hospital from January 2016 to August 2018,including 60 patients with diabetic retinopathy(DR),34 patients with non-proliferative retinopathy(NPDR)and 26 patients with proliferative retinopathy(PDR).150 patients without retinopathy were selected as DM group and 100 healthy people were selected as control group.Western blot was used to detect the expression of PERK,CHOP and IRE in retina.Results:The relative expression of serum PERK and IRE in DR group were(1.382±0.112)and(0.880±0.102),which were significantly higher than those in the control group and DM group(P<0.05).The relative expression of CHOP in serum of DR group was(1.011±0.110),which was significantly higher than that of the control group(P<0.05),there was no statistically significant difference with DM group(P>0.05).The relative expressions of PERK and IRE in PDR patients in DR group were(1.404±0.110)and(0.922±0.104),which were significantly higher than those in NPDR patients(P<0.05).There was no statistically significant difference in the relative expression of CHOP between NPDR and PDR patients in DR group(P>0.05).In DR group,PERK was positively correlated with IRE(r=0.402,P<0.05),while CHOP were not significantly correlated with PERK and IRE(P>0.05).Conclusion:The expressions of PERK,CHOP and IRE in patients with diabetic retinopathy are significantly increase,which may play an important role in the process of diabetic retinopathy.