环丙沙星对血管平滑肌细胞表型转换的调控作用

Effect of ciprofloxacin on regulating phenotypic transformation of vascular smooth muscle cells

目的研究环丙沙星(ciprofloxacin,CPFX)是否调控血管平滑肌细胞(vascular smooth muscle cells,VSMCs)表型转换。方法组织贴壁法分离,培养和鉴定来源于人体的主动脉VSMCs。通过CCK-8检测CPFX干预最佳浓度和时间。随后将VSMCs分为4组:对照组、CPFX组、血管紧张素Ⅱ(angiotensin Ⅱ,Ang Ⅱ)组、CPFX+Ang Ⅱ组。通过蛋白免疫印迹(Western blot)检测表型转换相关蛋白表达水平。结果 CPFX可引起平滑肌肌动蛋白α(α-smooth muscle actin,α-SMA)、平滑肌蛋白22(smooth muscle 22,SM22)、钙调节蛋白(calponin)表达水平降低,骨桥蛋白(osteopontin,OPN)表达水平升高。CPFX+Ang Ⅱ较单用CPFX作用更加显著。结论 CPFX参与调控VSMCs表型转换。

Objectives To investigate whether ciprofloxacin(CPFX)regulates the phenotypic transformation of vascular smooth muscle cells(VSMCs).Methods VSMCs were isolated,cultured and identified from human aorta by tissue adherent method.CCK-8 was used to detect the optimal concentration and time of CPFX intervention.Then VSMCs were divided into 4 groups:control group,CPFX group,angiotensinⅡ(AngⅡ)group,CPFX+AngⅡgroup.Western blot was used to detect the expression of phenotypic transformation related proteins.Results CPFX could decrease the expression of smooth muscle actinα(α-SMA),smooth muscle 22(SM22)and calponin and increase the expression of osteopontin(OPN)in VSMCs.The effect of CPFX+AngⅡwas more significant than that of CPFX alone.Conclusions CPFX is involved in the regulation of phenotypic transformation in VSMCs.