miR-590-5p对PDGF诱导气道平滑肌细胞增殖和迁移的作用和机制

Effects and mechanism of miR-590-5p on the proliferation and migration of airway smooth muscle cell induced by PDGF

目的探讨微小RNA-590-5p(miR-590-5p)在人血小板来源生长因子(PDGF)诱导的气道平滑肌细胞(ASMCs)中的作用以及潜在机制。方法ASMCs随机分为对照组(不做处理),模型组(用40 ng·m L-1PDGF刺激24h),和实验组(转染miR-590-5p模拟物后用40 ng·m L-1PDGF刺激24 h)。以细胞计数试剂盒-8(CCK-8)检测ASMCs的增殖;细胞划痕愈合实验检测ASMCs的迁移;以蛋白印迹法检测各组ASMCs中酪氨酸激酶2(JAK2)的表达水平。结果对照组、模型组、实验组细胞增殖率分别为(57.28±4.39)%,(84.38±3.45)%,(60.86±4.40)%;细胞迁移率分别为(19.27±2.49)%,(38.16±5.32)%,(24.68±3.04)%;JAK2的相对表达分别为0.69±0.08,2.19±0.13,1.26±0.11。模型组与对照组比较,实验组与模型组比较,差异均有统计学意义(均P<0.05)。结论miR-590-5p在PDGF诱导的ASMCs表达下调,miR-590-5p通过靶向JAK2抑制PDGF诱导的ASMCs的增殖和迁移。

Objective To investigate the role and potential mechanism of microRNA-590-5p(miR-590-5p)in human platelet-derived growth factor(PDGF)-induced airway smooth muscle cells(ASMCs).Methods ASMCs were randomly divided into a control group(without any treatment),a model group(stimulated with 40 ng·m L-1PDGF for24 h),and an experimental group(stimulated with 40 ng·m L-1PDGF for 24 h after the transfection of miR-590-5p mimics).The proliferation of ASMCs was detected using CCK-8.The migration of ASMCs was detected by cell scratch wound healing assay.The expression level of Janus kinase 2(JAK2)in the ASMCs of each group was detected by Western blot.Results The migration rates at 48 h of ASMCs in the control group,model group and experimental group were(57.28±4.39)%,(84.38±3.45)%and(60.86±4.40)%,respectively;the migration rates at were(19.27±2.49)%,(38.16±5.32)%and(24.68±3.04)%,respectively;the relative expressions of JAK2 were 0.69±0.08,2.19±0.13 and 1.26±0.11,respectively.The differences between the model group and the control group,as well as between the experimental group and the model group were statistically significant(P<0.05).Conclusion MiR-590-5p is down-regulated in PDGF-induced ASMCs,and miR-590-5p inhibits the proliferation and migration of ASMCs induced by PDGF by targeting JAK2.