胃癌患者血浆PAX5和RNF180基因甲基化情况及其临床意义

Methylations and clinical significances of plasma PAX5 and RNF180 genes in patients with gastric cancer

目的观察胃癌患者血浆配对盒蛋白5(paired box protein 5,PAX5)与环指蛋白180(ring finger protein 180,RNF180)基因甲基化情况,探讨其与临床病理特征及预后的关系。方法110例胃癌患者为胃癌组,同期体检健康者50例为对照组,采用甲基化特异性PCR法检测血浆PAX5与RNF180基因甲基化状态,分析其与胃癌患者临床病理特征的关系。随访3年,采用Kaplan-Meier生存曲线分析PAX5、RNF180甲基化与患者预后的关系。结果胃癌患者血浆PAX5、RNF180基因启动子区甲基化发生率(70.00%、62.73%)高于对照组(32.00%、30.00%)(P<0.05),胃癌组TNM分期Ⅰ~Ⅱ期患者血浆PAX5、RNF180基因甲基化发生率(65.17%、57.30%)低于TNM分期Ⅲ~Ⅳ期患者(90.48%、85.71%)(P<0.05),胃癌组男性与女性、年龄<55岁与≥55岁、肿瘤直径<2.5 cm与≥2.5 cm、细胞低-中分化与高分化及有无淋巴结转移患者血浆PAX5、RNF180基因甲基化发生率比较差异均无统计学意义(P>0.05)。发生PAX5基因甲基化的胃癌患者3年生存率(68.83%)低于未发生甲基化患者(84.85%)(P<0.05),发生RNF180基因甲基化的胃癌患者3年总体生存率(73.91%)与未发生甲基化胃癌患者(73.17%)比较差异无统计学意义(P>0.05)。结论胃癌患者血浆PAX5与RNF180基因甲基化发生率增高,与肿瘤TNM分期有关,发生PAX5基因甲基化的患者3年总生存率低。

Objective To observe the methylations of paired box protein 5(PAX5)and ring finger protein 180(RNF180)genes in the plasma of patients with gastric cancer,and to analyze their relationships with clinicopathological characteristics and prognosis.Methods The methylation-specific PCR method was used to detect the methylations of plasma PAX5 and RNF180 in 110 gastric cancer patients(gastric cancer group)and 50 healthy volunteers(control group),and their relationships with the clinicopathological characteristics of gastric cancer were analyzed.After 3 years of follow-up,Kaplan-Meier survival curve was used to analyze the relationships of PAX5 and RNF180 methylation with the prognosis.Results The methylation rates in the promoter regions of the plasma PAX5 and RNF180 genes were higher in gastric cancer group(70.00%,62.73%)than those in control group(32.00%,30.00%)(P<0.05),and were lower in TNM stageⅠ-Ⅱpatients in gastric cancer group(65.17%,57.30%)than those in TNM stageⅢ-Ⅳpatients(90.48%,85.71%)(P<0.05).There were no significant differences in the methylation rates of plasma of PAX5 and RNF180 genes between male and female patients,between patients aged<55 years old and≥55 years old,tumor diameter of<2.5 cm and≥2.5 cm,with low/moderate and high differentiation,and with and without lymph node metastasis(P>0.05).The 3-year survival rate of gastric cancer with PAX5 methylation(68.83%)was lower than that of patients without methylation(84.85%)(P<0.05),and the total 3-year survival rate showed no significant difference between patients with and without methylation of RNF180(73.91%vs.73.17%)(P>0.05).Conclusion The methylation rates of plasma PAX5 and RNF180 genes increase in patients with gastric cancer,which is correlated with the tumor TNM stages.The 3-years survival rate is low in patients with methylations of plasma PAX5 gene.