p16、p53和Ki-67蛋白在子宫颈鳞状上皮内病变表达及其意义

The Application value of p16,p53 and Ki-67 in cervical squamous intraepithelial lesion

目的探讨p16、p53和增殖细胞核蛋白(Ki-67)在子宫颈鳞状上皮内病变中的表达及应用价值。方法采用免疫组化SP法检测子宫颈反应性增生、低级别鳞状上皮内病变(LSIL)、高级别鳞状上皮内病变(HSIL)和子宫颈鳞癌中p16、p53和Ki-67蛋白的表达。结果p16、p53在子宫颈反应性增生组织中阴性表达,Ki-67蛋白则少量表达;p16、p53和Ki-67蛋白在LSIL、HSIL和子宫颈鳞癌组织中均明显表达,且随着病变的级别升高,三者的表达阳性率逐渐增高,差异有统计学意义(P<0.05);p16、p53和Ki-67蛋白在鳞状上皮组织中的表达存在分层现象;三者在LSIL、HSIL和子宫颈鳞癌组织中着色强阳性(++~+++)中的表达,差异有统计学意义(P<0.05)。结论p16、p53和Ki-67蛋白的表达在子宫颈鳞状上皮内病变的病理诊断、鉴别诊断及指导临床治疗方面有较高应用价值,可显著提高子子宫颈鳞状上皮内病变的早期诊断的准确率。

Objective To inveseigate the expression and application value of p16,p53 and Ki-67 in the cervical squamous intraepithelial lesion.Methods The immunehistochemical SP was used to detect the expression of p16,p53 and Ki-67 in cervical reactive hyperplasia,low-grade squamous intraepithelial lesion(LSIL),high-grade squamous intraepithelial lesion(HSIL)and cervical squamous cell carcinoma.Results The expression of p16,p53 were negative in cervical reactive hyperplasia,and the expression of Ki-67 was slight.The expression of p16,p53 and Ki-67 were positive expression in the tissue of LSIL、HSIL and cervical squamous cell carcinoma,and as the grade of SIL rose,the positive rates of p16,p53 and Ki-67 increased gradually(P<0.05).The expression of p16 and Ki-67 in squamous epithelial tissue had the stratification.The strongly positive expression of p16,p53 and Ki-67 in LSIL、HSIL and cervical squamous cell carcinoma had significant difference(P<0.05).Conclusions The expression of p16,p53 and Ki-67 protein has high application value in the pathological diagnosis,differential diagnosis and clinical treatment of cervical intraepithelial lesions,which can significantly improve the early diagnosis accuracy of cervical intraepithelial lesions.