基于网络药理学探讨沉香治疗腹痛的作用机制

Mechanism of Chenxiang in Abdominal Pain Based on the Network Pharmacology

目的:采用网络药理学方法筛选沉香的化学成分和作用靶点,分析沉香治疗腹痛相关疾病可能存在的作用机制。方法:通过中药系统药理数据库(TCMSP)检索沉香所有化学成分并筛选作用靶点,分别检索GeneCards和OMIM数据库筛选腹痛相关基因。利用Cytoscape软件进行网络合并,筛选核心网络。进一步采用蛋白互作网络(PPI)、基因GO功能富集和KEGG通路富集分析等探索可能涉及的分子机制。结果:共计筛选出符合要求的沉香有效成份8个,疾病相关靶点214个,药物相关靶点87个。最终共筛查到69个基因,分别富集于100个功能和表皮生长因子受体(EGFR)酪氨酸激酶抑制剂耐药性、内分泌抵抗、铂抗药性、ErbB信号通路以及MAPK信号通路5条信号通路。结论:沉香可能通过EGFR酪氨酸激酶抑制剂耐药性、内分泌抵抗、铂抗药性、ErbB信号通路以及MAPK信号通路治疗腹痛。

Objective:To screen the chemical constituents and targets of Chenxiang using network pharmacological method,so as to explore potential mechanism of Chenxiang in treatment of abdominal pain related diseases.Methods:Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)was used to retrieve the chemical constituents of Chenxiang and screen their targets.GeneCards and Online Mendelian Inheritance in Man(OMIM)databases were used to screen abdominal pain related genes.Cytoscape software was used to merge the network and screen the core.The possible molecular mechanisms were further explored by protein-protein interaction network(PPI),gene GO functional enrichment and KEGG pathway enrichment analysis.Results:A total of 8 active ingredients,214 disease-related targets and 87 drug-related targets were screened out.There were 69 genes enriched in 100 functions and 5 signaling pathways,including epidermal growth factor receptor(EGFR)tyrosine kinase inhibitor resistance,endocrine resistance,platinum drug resistance,ErbB and MAPK signaling pathway.Conclusion:Chenxiang may cure abdominal pain through EGFR tyrosine kinase inhibitor resistance,endocrine resistance,platinum resistance,ErbB and MAPK signaling pathway.